稀土Yb对NIH3T3细胞生长的影响  

Effect of Yb^(3+) on Growth of NIH3T3 Cells

作　　者：张丽平[1,2] 杨频[2] 丁庆伟[1] 陈明[3] 

机构地区：[1]太原科技大学环境科学教研室,太原030024 [2]山西大学分子科学研究所,太原030006 [3]山东省泰山医学院生命科学研究所,山东泰安271000

出　　处：《太原科技大学学报》2009年第3期264-267,共4页Journal of Taiyuan University of Science and Technology

基　　金：山西省自然科学青年基金(2007021010)

摘　　要：应用MTT方法,研究了稀土元素Yb对小鼠成纤维细胞生长的影响。以体外传代培养的细胞株NIH3T3为研究对象,给予Yb3+(0.001 mmol/L、0.01 mmoL/L、0.1和1.00 mmol/L)培养1d后,观察NIH3T3细胞的生长情况,同时,应用MTT法检测稀土元素Yb对NIH3T3细胞生长的作用。结果发现稀土Yb对NIH3T3细胞的生长具有明显的抑制作用(P<0.05),且随着Yb3+剂量的增加,对该细胞生长的抑制作用明显增强(P<0.05)。这些结果提示稀土Yb具有体外抗纤维化的作用,有望应用于组织纤维化疾病的治疗上。Using MTT,the effect of Yb^3＋ on the growth of NIH3T3 cells was studied. The histological changes of NIH3T3 cells were observed on the first day after treated with different doses （0.001,0.01,0.10 and 1.00 mmol·L^-1） of Yb^3= in vitro. At the same time,the proliferation of NIH3T3 cells were detected by MTT. The results showed that the evident inhibition of Yb^3＋ to NIH3T3 cells growth occurred after treatment with Yb^3＋ （ P 〈 0.05） and was gradually strengthened following the increase of the dose of Yb^3＋ （ P 〈 0.05 ）. These results show that ytterbium had effect of anti-fibrosis in vitro and expected to use in therapy of tissue fibrosis.

关 键 词：NIH3T3 成纤维细胞 稀土离子 MTT法 

分 类 号：O614.33[理学—无机化学]