麝香保心丸对自发性高血压大鼠肾脏炎症状态的改善作用  

Anti-inflammatory Effects of Heart-protecting Musk Pill on Kidney of Spontaneously Hypertensive Rat

作　　者：田登科[1] 陈刚领[1] 李亚娟[1] 胡送友[1] 刘俊[1] 卞卡[1,2] 

机构地区：[1]上海中医药大学穆拉德中药现代化研究中心,上海201203 [2]美国德克萨斯大学休斯顿医学院综合生物及药理学系,德克萨斯大学分子医学研究所

出　　处：《辽宁中医药大学学报》2009年第6期5-8,共4页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：国家科技部十一五支撑计划(2006BAI11B08-03);上海市科委基础研究重点项目(05JC14056)

摘　　要：目的:观察麝香保心丸(HMP)对自发性高血压大鼠(SHR)肾脏炎症状态的影响,探讨其对高血压肾脏疾病的防治作用及机制。方法:将6周龄SHR分为HMP治疗组和高血压模型对照组,以同周龄的Wistar-Kyoto(WKY)大鼠为正常对照,在3个时间点(给药6周,给药14周,停药9周)研究HMP对SHR血压值、肾脏炎性因子表达和氧化应激水平的影响。结果:在整个实验过程中HMP对SHR无明显降压作用(P>0.05)。HMP在mRNA和蛋白水平抑制了细胞间粘附分子-1(ICAM-1)和诱导型一氧化氮合酶(iNOS)在SHR肾脏的高表达(P<0.05),在mRNA水平抑制了肿瘤坏死因子-α(TNF-α)的高表达(P<0.05)。HMP在给药14周和停药9周时降低了过氧化物酶体增殖物活化受体γ(PPARγ)的mRNA表达(P<0.05),而在给药6周和给药14周时明显降低了PPARγ的蛋白表达(P<0.05)。HMP给药14周及停药9周后均可显著提高SHR肾脏组织总抗氧化能力(P<0.05),给药14周时能显著降低SHR肾脏组织蛋白羰基化水平(P<0.05)。结论:HMP给药后能够显著减轻SHR肾脏炎症反应,并且伴随着肾脏氧化应激水平的降低,这可能是HMP在防治高血压肾病方面具有应用潜能的作用机理。Objective： To investigate the effect of Heart-protecting Musk Pill （ HMP ）on inflammatory status of the kidney from spontaneously hypertensive rat （SHR）, and to explore preventive and therapeutic usage of HMP on hypertensive nephropathy. Methods： SHR of 6 weeks old were divided into HMP group,high blood pressure model group, and-age-matched Wistar-Kyoto rats were used as normal control. The effects of HMP on systolic blood pressure, expression of inflammation-related factors and level of oxidative stress were determined at 3 time points： 6 weeks of treatment; 14 weeks of treatment; and 9 weeks post-treatment. Results： HMP did not inference the elevation of blood pressure of SHR through period of treatment（P〉0.05）.However, the TCM compound inhibited the expression of intercellular adhesion molecule-1 （ICAM-1） and inducible nitric oxide synthase （iNOS） at both mRNA and protein levels （P〈0.05）. HMP also inhibited the mRNA expression of turner nerosis factor-α （TNF-α） in SHR kidney （P〈0.05）. At the time points of 14 weeks of treatment and 9 weeks post-treatment, HMP significantly attenuated peroxisome proliferatoractivated receptor γ/（PPAR γ ） mRNA expression（P〈0;05）. The protein level of PPAR γ was also inhibited by HMP markedly after 6 and 14 weeks of treatment（P〈0.05）. After 14 weeks of treatment, HMP increased total anti-oxidant capacity of renal tissue from SHR （P〈0.05） and this elevated anti-oxidant capacity was even maintained for 9 weeks post-treatment （P〈0.05）. In addition, HMP 14 week of treatment significantly reduced protein carbonyl contents of SHR kidney （P〈0.05）. Conclusion： HMP can improve the inflammatory status of SHR kidney independent of interfering with blood pressure. The anti-inflammatory effect of HMP was also acccmpanied by the decreasing level of oxidative stress, which may serve base for the advanced therapeutic utility of HMP in hypertensive renal disease.

关 键 词：麝香保心丸 自发性高血压大鼠 高血压肾脏疾病 炎症反应 

分 类 号：R285.5[医药卫生—中药学]