全蝎 蜈蚣 土鳖虫 僵蚕4种药材商品中总磷脂的含量测定  

Total Phospholipids Determination Four Kings of Chinese Herbal Products

作　　者：乔歌[1] 李峰[1] 张智华[1] 

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600

出　　处：《辽宁中医药大学学报》2009年第6期225-227,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：辽宁省教育厅;沈阳市科技局资助课题(20211473)

摘　　要：目的:建立全蝎、蜈蚣、土鳖虫、僵蚕等4种药材中总磷脂含量测定方法。方法:采用Folch试剂超声提取,钼蓝试剂显色,分光光度法测定10个不同地区的全蝎等4种药材中总磷脂的含量。结果:样品中总磷脂含量以磷计的回归方程Y=0.0238X-0.01(r=0.9994),线性范围在2.38～7.14μg,RSD=0.53%,平均回收率为99.9(%n=5)。结论:采用钼蓝比色法测定全蝎等4种药材中10个不同地区商品中总磷脂含量,方法简便,方法学结果理想,可作为该4种药材质量控制的方法。Objective：To establish the method for determining the content of total phospholipids in Scorpio, Scolopendra,Eupolyphaga seu Steleophaga ,Bombyx Batryticatus and evaluate the quality of four medicinal materils from different collecting area. Methods： After extracting by folch agent,Moly-blue agent display color and adopting Ui-vis to determine the content of total phospholipids in four medicinal materils from ten different collecting areas. Resuhs： The linear equation of phosphonium is A=0.0238 C - 0.01 （r=0.9994）,the range of linearity from 2.38 to7.14 μg,RSD=0.53%,the average recovery was 99.9%. Conclusion： The above method to determine the content of total phospholipids in four medicinal materils is brief , both recur and precision density are all right,it can pilot quality control of the four medicinal materils.

关 键 词：全蝎 蜈蚣 总磷脂 含量测定 钼蓝比色法 

分 类 号：R284.1[医药卫生—中药学]