冰冻储存对淡色库蚊非特异性酯酶活性的影响  

Effect of frozen storage on non-specific esterase activity of Culex pipiens pallens

作　　者：李士根[1] 王霄[2] 谭文彬[1] 甄天民[3] 全芯[1] 高红刚[1] 刘永春[1] 

机构地区：[1]济宁医学院寄生虫学教研室,山东济宁272013 [2]济宁医学院外总教研室 [3]山东省医学科学院

出　　处：《中国媒介生物学及控制杂志》2009年第3期187-188,共2页Chinese Journal of Vector Biology and Control

基　　金：山东省卫生厅资助项目(2001CA2CEB1)

摘　　要：目的探讨冰冻储存对淡色库蚊非特异性酯酶(non-specific esterase,NSE)活性的影响,为蚊虫抗药性生物化学检测方法的推广应用提供依据。方法以β-乙酸萘酯为底物,坚固蓝B盐为显色剂,测定单个蚊虫NSE活性。结果DDVP抗性品系淡色库蚊NSE活性水平明显高于敏感品系;经完全随机设计的方差分析比较,-20℃冰冻0、7、14、21、28d的淡色库蚊NSE活性A值之间的差别,敏感品系4龄幼虫的F值为0.7642,该品系3日龄雌成虫的F值为0.7452,DDVP抗性品系4龄幼虫的F值为0.1092,该品系3日龄雌成虫的F值为0.1521,P值均>0.05,差异无统计学意义。结论-20℃冰冻28d对淡色库蚊4龄幼虫和3日龄雌成虫体内NSE活性无影响,可在现场采集蚊虫带回实验室集中测定。Objective To explore the effect of frozen storage on non-specific esterase （NSE） activity of Culex pipiens pallens and provide a basis for the generalization and application of insecticide-resistance detection of mosquito by biochemical method. Methods The NSE activity of single mosquito was determined based on β-naphthalene acetic acid ester （β-naphthyl acetate, β-NA） as substrate and solid blue B salt （fast blue B salt） as developer. Results The NSE activity of DDVP-resistant strain was obviously higher than that of susceptible strain. For the A value of NSE activity from Cx.pipiens pallens after frozen for 0, 7, 14, 21 and 28 days at -20 ℃, the F value of 4 instar larvae of susceptible strain mosquito was 0.7642 by the complete randomized design variance comparison and the F value of 3-day-old adult female of susceptible strain mosquito was 0.7452. The F value of 4 instar larvae of DDVP resistant strain mosquito was 0.1092, and the F value of 3-day-old adult female of DDVP- resistant strain mosquito was 0.1521. All of the P value were greater than 0.05 and no significant difference was found. Conclusion There was no difference between on the NSE activity of 4 instar larvae and 3-day-old aduh female of Cx.pipiens pallens after frozen for 28 days, and mosquitoes collected from the field could be taken to the lab for batch determination.

关 键 词：淡色库蚊 非特异性酯酶 抗药性 

分 类 号：R384.1[医药卫生—医学寄生虫学]