Plant polar growth in tobacco disturbed by c-tubulin gene silencing  

作　　者：Shuang Zhao Kun Yang Qian Ma Qi Wang Xiaodan Wang Yanhong Li 

机构地区：[1]College of Life Science, Capital Normal University, Beijing 100048, China

出　　处：《Progress in Natural Science:Materials International》2009年第6期685-691,共7页自然科学进展·国际材料（英文版）

基　　金：supported by the Beijing New Star of Science and Technology Project (H013610020112)

摘　　要：To further understand the functions of γ-tubulin in plant cells, we conducted a study in which the γ-tubulin gene was down-regulated in tobacco plants （obtained by the Agrobacterium-mediated method）. This involved transforming the target fragments, in which the sense and antisense partial γ-tubulin cDNA fragments were ligated together, into Nicot＆na tabacum var. Samsun NN. The γ-tnbulin downregulated transformants developed multiple meristems or branches with trumpet-shaped leaves; their root generation also appeared abnormal, with the taproots undeveloped, whereas lateral roots were developed. In addition, the content of indole-3-acetic acid （IAA） and expression of polarity transportation vector PGP1 were aberrant. These results suggest that γ-tubulin gene silencing disturbed the polar growth of tobacco plants, and that this phenomenon was probably correlated with the IAA content and the polar transportation process.To further understand the functions of c-tubulin in plant cells, we conducted a study in which the c-tubulin gene was down-regulated in tobacco plants (obtained by the Agrobacterium-mediated method). This involved transforming the target fragments, in which the sense and antisense partial c-tubulin cDNA fragments were ligated together, into Nicotiana tabacum var. Samsun NN. The c-tubulin down-regulated transformants developed multiple meristems or branches with trumpet-shaped leaves; their root generation also appeared abnormal, with the taproots undeveloped, whereas lateral roots were developed. In addition, the content of indole-3-acetic acid (IAA) and expression of polarity transportation vector PGP1 were aberrant. These results suggest that c-tubulin gene silencing disturbed the polar growth of tobacco plants, and that this phenomenon was probably correlated with the IAA content and the polar transportation process.

关 键 词：TOBACCO Γ-TUBULIN Gene silencing Polar growth 

分 类 号：Q959.117[生物学—动物学] Q942