Maternal inheritance in polyploid fish inferred from mitochondrial ATPase genes analysis  

作　　者：Jinpeng Yan 1, Xinhong Guo1, Shaojun Liu, Jun Xiao, Zhen Liu, Yubao Chen, Yun Liu Key Laboratory of Protein Chemistry and Fish Developmental Biology of Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China 

出　　处：《Progress in Natural Science:Materials International》2009年第6期693-698,共6页自然科学进展·国际材料（英文版）

基　　金：supported by the National Natural Science Foundation of China (Grant Nos.30725028,30330480 and 30571444);the National Basic Research Program of China (Grant No.2007CB109206);the Specialized Research Fund for the Doctoral Station of University of the Education Ministry of China (Grant No.200405422001);the Program for Changjiang Scholars and Innovative Research Team in University of Education Ministry of China (Grant No.IRT0445);the Agriculture Technological Production Foundation of the Science and Technology,Ministry of China (Grant No.2005380);the Key Item of Science and Technology of Hunan Province (Grant No.2006NK2008)

摘　　要：The sequences of the ATPase8/6 genes for the triploid, tetraploid and pentaploid hybrids as well as for their male parent blunt snout bream were determined. In order to examine mitochondrial maternal inheritance, the sequences were subjected to a comparative sequence analysis with the homologous sequences of red crucian carp, their female parent, and zebrafish as the outgroup. Base composition and variation as well as the divergences based on nucleotide sequences and deduced amino acid sequences were calculated. Phylogenetic trees were also constructed with maximum parsimony (MP), minimum evolution (ME), neighbor joining (NJ) and the unweighted pair group method with arithmetic mean (UPGMA) algorithms in MEGA 3.1. The results showed that most nucleotide substitutions occurred at the third codon position of the two genes and thus represented synonymous mutations. The nucleotide sequence divergences of the ATPase8/6 genes ranged from 0.0% to 21.6% among ingroup samples (three types of polyploids and their parents), and 27.0-28.2% between their ingroup and the outgroup samples. All the polyploids were considerably closer in sequence relationship to the female parent red crucian carp (0.0-3.3%) compared to their male parent blunt snout bream (21.0-21.6%). The phylogenetic trees also showed a similar result. In conclusion, the mitochondrial ATPase8/6 genes of artificial polyploid fish stringently indicated maternal inheritance. Our results also suggested that the ATPase8/6 genes are valuable genetic markers to track genealogies and variations in the progenies of the hybrids.The sequences of the ATPase8/6 genes for the triploid, tetraploid and pentaploid hybrids as well as for their male parent blunt snout bream were determined. In order to examine mitochondrial maternal inheritance, the sequences were subjected to a comparative sequence analysis with the homologous sequences of red crucian carp, their female parent, and zebrafish as the outgroup. Base composition and variation as well as the divergences based on nucleotide sequences and deduced amino acid sequences were calculated. Phylogenetic trees were also constructed with maximum parsimony （MP）, minimum evolution （ME）, neighbor joining （NJ） and the unweighted pair group method with arithmetic mean （UPGMA） algorithms in MEGA 3.1. The results showed that most nucleotide substitutions occurred at the third codon position of the two genes and thus represented synonymous mutations. The nucleotide sequence divergences of the ATPase8/6 genes ranged from 0.0% to 21.6% among ingroup samples （three types of polyploids and their parents）, and 27.0-28.2% between their ingroup and the outgroup samples. All the polyploids were considerably closer in sequence relationship to the female parent red crucian carp （0.0-3.3%） compared to their male parent blunt snout bream （21.0-21.6%）. The phylogenetic trees also showed a similar result. In conclusion, the mitochondrial ATPase8/6 genes of artificial polyploid fish stringently indicated maternal inheritance. Our results also suggested that the ATPase8/6 genes are valuable genetic markers to track genealogies and variations in the progenies of the hybrids.

关 键 词：Maternal inheritance ATPase genes Triploid hybrids Tetraploid hybrids Pentaploid hybrids 

分 类 号：S917.4[农业科学—水产科学]