Identification of three major Bemisia tabaci biotypes in China based on morphological and DNA polymorphisms  

作　　者：Baoli Qiu Yanping Chen Li Liu Weilu Peng Xiaoxi Li Muhammad Z. Ahmed Vartika Mathur Yuzhou Du Shunxiang Ren 

机构地区：[1]Department of Entomology, South China Agricultural University, Cruangzhou 510640, China [2]Yantai Entry-Exit Inspection and Quarantine Bureau, Yantai 264000, China [3]Department of Zoology, Sri Venkateswara College, University of Delhi, Delhi 110021, India [4]Department of Plant Protection, Yangzhou University, Yangzhou 225009, China

出　　处：《Progress in Natural Science:Materials International》2009年第6期713-718,共6页自然科学进展·国际材料（英文版）

基　　金：supported by the National Natural Science Foundation of China (Grant Nos.30400292 and 30671732);the National Basic Research Program of China (2006CB102005 and 2009CB119203)

摘　　要：The sweet potato whitefly, Bemisia tabaci, is a complex species, and many of its biotypes are important agricultural pests. The B and Q biotypes are invasive and coexist with the native Cv biotype on vegetable and ornamental crops in China. In this study, these three biotypes were identified based on their morphological characteristics, RAPD-PCR analysis, and DNA sequences of the CO1 gene. The anterior and posterior wax fringes of the B, Q, and Cv biotypes significantly differed from each other. Based on this morphological characteristic, the three biotypes can easily be distinguished in greenhouses and fields. Genomic DNA RAPD-PCR band patterns also revealed differences between these biotypes using the H16 primer. The B biotype has three bright DNA bands between 250 and 600 bp, the Q biotype only has one bright band at -300 bp, while the Cv biotype has no band between 250 and 500 bp. Both the Cv and Q biotypes have two bright bands at -750 and 1000 bp while the B biotype has only one band at -1000 bp. Based on the COI gene, the genetic identity between B and Cv, B and Q, and Q and CV was 85.8%, 94.7%, and 86.0%, respectively. The MP tree indicated that the phylogenetic relationship between the B and Q biotypes is much closer than that between the B and Cv or the Q and Cv biotypes. The uses of the morphological, RAPD-PCR, and DNA sequencing methods in biotype identification of B. tabaci are discussed.The sweet potato whitefly, Bemisia tabaci, is a complex species, and many of its biotypes are important agricultural pests. The B and Q biotypes are invasive and coexist with the native Cv biotype on vegetable and ornamental crops in China. In this study, these three bio- types were identified based on their morphological characteristics, RAPD-PCR analysis, and DNA sequences of the COI gene. The ante-rior and posterior wax fringes of the B, Q, and Cv biotypes significantly differed from each other. Based on this morphological characteristic, the three biotypes can easily be distinguished in greenhouses and fields. Genomic DNA RAPD-PCR band patterns also revealed differences between these biotypes using the H16 primer. The B biotype has three bright DNA bands between 250 and 600 bp, the Q biotype only has one bright band at 300 bp, while the Cv biotype has no band between 250 and 500 bp. Both the Cv and Q biotypes have two bright bands at 750 and 1000 bp while the B biotype has only one band at 1000 bp. Based on the COI gene, the genetic identity between B and Cv, B and Q, and Q and CV was 85.8%, 94.7%, and 86.0%, respectively. The MP tree indicated that the phylogenetic relationship between the B and Q biotypes is much closer than that between the B and Cv or the Q and Cv biotypes. The uses of the morphological, RAPD-PCR, and DNA sequencing methods in biotype identification of B. tabaci are discussed.

关 键 词：Bemisia tabaci complex BIOTYPE DNA polymorphism IDENTIFICATION MORPHOLOGY 

分 类 号：S646[农业科学—蔬菜学] Q987[农业科学—园艺学]