实时荧光PCR技术检测登革病毒的研究  

Research of Real-Time Fluorescence PCR Detection on Dengue Virus

作　　者：姚锦绣[1,2] 赵卫[1] 张玲[1] 李玉静[1] 林培政[3] 

机构地区：[1]南方医科大学公共卫生与热带医学学院病毒研究所,广州510515 [2]阳江市人民医院检验科,529500 [3]广州中医药大学,510006

出　　处：《华西医学》2009年第4期912-914,共3页West China Medical Journal

基　　金：广东省医学科学技术研究基金(A2006382);国家自然科学基金(U0632009)

摘　　要：目的：建立登革病毒的荧光定量PCR检测技术。方法：针对1-4型登革病毒3′端非编码区的一段高度保守序列设计引物和探针,建立登革病毒实时荧光PCR检测方法及定量的检测方法。并对10例ELISA法检测为登革病毒阳性的病例,取其发热1-2天的临床血清标本进行荧光定量PCR检测。结果：实时荧光PCR检测1-4型登革病毒均为阳性,该探针和引物是登革病毒检测的通用探针和引物。实时荧光PCR检测10份临床血清,6份为阳性,阳性率为60%。结论：实时PCR方法是一个快速、特异性强、敏感性高的检测登革病毒的方法,适用于登革热的临床早期诊断,具有很好的社会效益和经济效益。Objective： Establishing the fluorescence quantitative PCR detection technology of dengue virus. Method： Using highly conserved sequence of 3 UTR in 1 - 4. types to design primers and probe for dengue virus, establishing the real-time fluorescent PCR method to detect and quantify the dengue virus. And using the method to test 10 clinical serum samples that ELISA assay has tested positive, which were collected from the fever patients of 1 to 2 days. Results： Using real-time fluorescence PCR to detect four types of dengue virus, all were positive. The probes and primers are universal ones for dengue virus detection. Using this method to detect 10 clinical sera, 6 were positive and the positive rate was 60%. Conclusion： Real-time PCR method is a fast, specific, sensitive detection method for dengue virus. It＇s applicable for the early clinical diagnosis of dengue fever and there are very good social and economic benefits.

关 键 词：登革病毒 TAQMAN探针 实时PCR 

分 类 号：R512.8[医药卫生—内科学]