尿液中违禁药物的载体促进传递液相微萃取-高效液相色谱法测定  

Determination of Illicit Drugs in Urine Based on Carrier-facilitated Liquid-liquid-liquid Microextraction Coupled with High Performance Liquid Chromatography

作　　者：刘庆艳[1] 罗芳苜[2] 

机构地区：[1]湖南环境生物职业技术学院,湖南衡阳421005 [2]衡阳财经工业职业技术学院,湖南衡阳421002

出　　处：《分析测试学报》2009年第5期589-591,596,共4页Journal of Instrumental Analysis

基　　金：湖南省教育厅科学研究项目资助(08D056);湖南省自然科学基金资助项目(06JJ2060)

摘　　要：建立了基于载体促进传递的液相微萃取-高效液相色谱法同时测定尿液中违禁药物吗啡、可待因和安非他明的方法。通过在有机相添加极性载体,扩大了液相微萃取的应用范围,对亲水性和疏水性的分析物实现了同时富集。考察了载体浓度、有机溶剂、萃取时间?搅拌速率对目标分析物富集因子的影响。在优化的实验条件下,方法的线性范围为0.1～5.0mg.L-1,相关系数大于0.996,检出限低至0.025mg.L-1,相对标准偏差小于9.2%。该方法用于实际尿样分析,结果满意。A method for the simultaneous determination of morphine, codeine and amphetamine in urine was developed based on carrier-facilitated liquid -liquid -liquid microextraction （LLLME） cou- pled with high performance liquid chromatography. By adding a polar carrier in the organic phase, the simultaneous extraction and enrichment of hydrophilic （ morphine and codeine） and hydrophobic （amphetamine） drugs were achieved. The study has expanded the application of LLLME to extract a hydrophilic substance from complex biological matrices. Effects of carrier concentration, organic sol- vent, extraction time and stirring rate on the em＇iehment factor of analytes were explored. Under opti- mal experimental conditions, the linear ranges for three analytes were from 0. 1 to 5.0 mg · L -1 with correlation coefficients（ r2 ） of higher than 0. 996. The detection limit was 0. 025 mg · L-1 （ S/N = 3 ） with relative standard deviations lower than 9.2%. This method was applied to determine the real u- rine specimens with satisfactory results.

关 键 词：载体促进 液相微萃取 高效液相色谱 违禁药物 吗啡 可待因 安非他明 

分 类 号：O657.72[理学—分析化学]