血管外膜损伤后血管内皮的功能及形态变化  

Changes of vascular endothelium in function and morphology after adventitial injury

作　　者：汤月霞[1,2] 梁春[1] 吴宗贵[1] 

机构地区：[1]上海长征医院心内科,上海200003 [2]济南军区青岛第一疗养院,山东青岛266071

出　　处：《心脏杂志》2009年第3期347-350,共4页Chinese Heart Journal

摘　　要：目的探讨血管内皮损伤在外膜损伤致内膜病变中的作用。方法选用纯种新西兰大白兔,采用胶原酶消化+机械分离的方法建立外膜损伤动物模型,HE染色后,观察外膜损伤血管的形态变化。采用Medlab-U/4C501生物信号采集处理系统检测血管内皮依赖性舒张功能,电镜下观察血管内皮细胞的微观形态变化。用实时PCR检测单核细胞趋化蛋白-1(MCP-1)mRNA的表达。结果外膜损伤后3 d血管内皮依赖性舒张功能减退及内皮细胞损伤,7 d出现内膜增生性病变。结论血管外膜损伤可能通过损伤血管内皮导致内膜病变的形成。AIM To explore the role of vascular endothelial injury in the intimal lesion caused by adventitial injury. METHODS Models of New Zealand rabbits with vascular adventitial injury were established by collogenase digestion and mechanical dissection. HE staining was used to detect the morphological changes in vessels after adventitial injury. Endothelial-dependent vasorelaxation function was detected with Medlab-U/4C501 biological signal collection and analysis system. Morphological changes in endo- thelial cells were observed under electron microscope and real-time PCR were used to determine the ex- pression of monocyte chemotactic protein-1 （MCP-1） mRNA. RESULTS Compared with that in control vessels, the endothelial-dependent vasorelaxation function was failing and endothelial cell injuries were found 3 days after injury. Expression of MCP-1 mRNA increased 3 days after injury and intimal hyperplasia was observed 7 days after injury. CONCLUSION Vascular adventitial injury may cause vascular endothelial injury and then lead to intimal lesion.

关 键 词：血管外膜损伤 内膜增生性病变 内皮依赖性舒张功能 兔 

分 类 号：R543.5[医药卫生—心血管疾病]