ELISA、PA法和胶体硒法在抗-HIV检测中的应用  

Application of ELISA and PA Method in Anti-HIV Detection

作　　者：陈敏[1] 

机构地区：[1]江苏省常州市疾病预防控制中心,213003

出　　处：《职业与健康》2009年第12期1265-1266,共2页Occupation and Health

摘　　要：目的为了提高HIV抗体的检测质量和速度,防止阳性标本的漏检。方法常州市疾病预防控制中心(CDC)对220份HIV抗体需复检的血清标本用酶联免疫吸附试验(ELISA)法、明胶凝集法(PA法)和胶体硒法同时进行复测,和江苏省CDC的确证试验结果比对。结果在检测的220份血清标本中,其中有116份血清标本的3项检测结果均为阴性,104份血清标本的确证试验结果为:HIV-1抗阳性93份,不确定的6份阴性的5份。结论在检测HIV抗体时,可以根据需要,将ELISA、PA和胶体硒法结合使用,以提高HIV抗体检测的阳性率。[ Objective ] To improve the quality and speed of HIV antibody test, and prevent undetected positive specimens. [ Methotis ] 220 serum samples of HIV antibody were retested simultaneously with ELISA, Particle Agglutination test （ PA ） and electroselenium method by Changzhou CDC, the results were compared with Jiansu CDC＇ confirmatory test results. [ Results ] Of 220 serum samples, 3 test results of 116 samples were negative, confirmatory test results of 104 samples were that 93 of HIV-1 anti-positive, 6 of uncertainty and 5 of negativity. [ Conclusion] In the detection of HIV antibodies, according to requirement, ELISA, PA and dectroselenium method can be used as a combination to improve the HIV antibody positive rate.

关 键 词：HIV抗体 ELISA法 PA法 胶体硒法 

分 类 号：R115[医药卫生—公共卫生与预防医学]