Stylet penetration behaviors of the cotton aphid Aphis gossypfi on transgenic Bt cotton  

作　　者：Kun Xue Xiao-Ying Wang Cui-Hong Huang Rong-Jiang Wang Biao Liu Feng-Ming Yan Chong-Ren Xu 

机构地区：[1]College of Life Sciences, Peking University, Beijing [2]College of Plant Protection, Henan Agricultural University, Zhengzhou [3]Nanjing Institute of Environmental Sciences, State Environmental Protection Administration of China, Nanjing [4]College of Life and Environmental Sciences, Minzu University of China, Beijing, China

出　　处：《Insect Science》2009年第2期137-146,共10页昆虫科学（英文版）

基　　金：Acknowledgments We thank W. F. Tjallingii, Wageningen University, the Netherlands, for the scientific and technical support on DC-EPG applications, and E. A. Backus, USDA Agricultural Research Service, Crop Diseases, Pests ＆ Genetics Unit, USA, for help and comments on statistical data analysis. This work was supported jointly by National Key Basic Research Program of China （No. G2000046803）, National Natural Science Foundation of China （No. 39970153） and Jiangsu Science Foundation （BK2006501）.

摘　　要：Stylet penetration behaviors of cotton aphids Aphis gossypii Glover on a transgenic cotton line ＂GK-12＂ expressing Bt toxic protein of Cry 1A （Bt cotton） and a non- Bt conventional cotton line ＂Simian-3＂ （CK cotton） were recorded with the direct current electrical penetration graph （DC-EPG） technique. Cotton aphids reared on Bt cotton （abbreviated as Bt-aphids） and its parental non-Bt control line （CK-aphids） for more than 20 generations each, were used for recordings on two cotton lines. Among 47 selected parameters reflecting the activities of aphid stylets within plant tissues, there were eight parameters of CK-aphids showing significant differences between the performances of CK- aphids on Bt cotton and CK cotton, while for Bt-aphids, all the parameters were statistically equal between the performances on the two cotton lines. All parameters with significant differences indicated that CK-aphids could penetrate into Bt cotton more easily, but the phloem saps of Bt cotton were not as good as those of regular cotton for CK-aphids. Based on the present results, we concluded that there were some factors in Bt cotton affecting penetration behaviors of CK-aphids, but it just took several generations for CK-aphids to completely adapt Bt cotton, and Bt-aphids could feed on two cotton lines without difficulty.Stylet penetration behaviors of cotton aphids Aphis gossypii Glover on a transgenic cotton line ＂GK-12＂ expressing Bt toxic protein of Cry 1A （Bt cotton） and a non- Bt conventional cotton line ＂Simian-3＂ （CK cotton） were recorded with the direct current electrical penetration graph （DC-EPG） technique. Cotton aphids reared on Bt cotton （abbreviated as Bt-aphids） and its parental non-Bt control line （CK-aphids） for more than 20 generations each, were used for recordings on two cotton lines. Among 47 selected parameters reflecting the activities of aphid stylets within plant tissues, there were eight parameters of CK-aphids showing significant differences between the performances of CK- aphids on Bt cotton and CK cotton, while for Bt-aphids, all the parameters were statistically equal between the performances on the two cotton lines. All parameters with significant differences indicated that CK-aphids could penetrate into Bt cotton more easily, but the phloem saps of Bt cotton were not as good as those of regular cotton for CK-aphids. Based on the present results, we concluded that there were some factors in Bt cotton affecting penetration behaviors of CK-aphids, but it just took several generations for CK-aphids to completely adapt Bt cotton, and Bt-aphids could feed on two cotton lines without difficulty.

关 键 词：Aphis gossypii Bt cotton electrical penetration graph feeding behavior non-target effects stylet penetration behavior 

分 类 号：S562[农业科学—作物学] TQ325.907[化学工程—合成树脂塑料工业]