硫化氢对离体大鼠心室肌细胞ATP依赖的钾通道外向电流的影响  

Effects of hydrogen sulfide on the KATP current in isolated rat ventricular myocytes

作　　者：李艳兵[1] 钟光珍[1] 杨新春[1] 刘秀兰[1] 

机构地区：[1]首都医科大学附属北京朝阳医院心脏中心,北京100020

出　　处：《中华心血管病杂志》2009年第5期445-448,共4页Chinese Journal of Cardiology

基　　金：国家自然科学基金资助项目（30770875）;北京市自然科学基金资助项目（7072027）;北京科技新星计划（2008B62）.

摘　　要：目的观察内源性及外源性硫化氢（hydrogen sulfide，H2S）对大鼠离体心室肌细胞ATP依赖的钾通道（KATP）外向电流的影响，以探讨H2S对心室肌细胞的作用。方法对大鼠离体心脏采用胶原酶酶解法得到单个心室肌细胞，采用膜片钳全细胞技术记录DL-propargylglycine（PPG）及不同浓度硫氢化钠（NaHS，外源性H2S的供体）干预前后的KATP电流。结果经PPG（200μmol／L）干预后，KATP峰电流密度（＋70mV）显著减小[干预前后分别为（5.3258±0.7556）pA／pF比（3.7856±0.4312）pA／pF，P〈0.01]，且具有时间依赖性。经NaHS（9.375、18.75、37.5、75、150μmol／L）干预后，KATP峰电流密度呈浓度依赖性增大，至150μmol／L时峰电流密度明显增大[（6.6310±0.6092）pA/pF比（9.0949±1.0259）pA／pF，P〈0.01]。结论内源性及外源性H2s均可以开放大鼠离体心室肌KATP通道，使KATp电流增加。Objective To investigate the effects of endogenous and exogenous hydrogen sulfide （H2S） on the KATP current in isolated rat ventricular myocytes. Methods Ventricular myocytes were isolated from rat heart by modified Langendoff perfusion with collagenase. KATP current of single rat ventricular myocytes was recorded by whole-cell patch-clamp technique. Results The density of KATp current was significantly reduced by 200μmol/L DL-propargylglycine（PPG, an irreversible inhibitor of the H2S） [（5.3258±0.7556 ） pA/pF vs. （3.7856±0.4312） pA/pF, P 〈 0.01] in a time-dependent way. The density of KATP current could be significantly increased by NariS（ a H2S donor, 9.375, 18.75, 37.5, 75, 150μmol/L） in a concentration-dependent manner [（6.6310± 0.6092）pA/pF vs. （9.0949±1.0259） pA/pF at 150μmol/L, P 〈 0.01]. Conclusion Both endogenous and exogenous H2S could open KATP channels and enhance the KATP current in rat ventricular myocytes.

关 键 词：硫化氢 钾通道 膜片钳术 

分 类 号：R686[医药卫生—骨科学]