p53-p21^waf1通路在口腔鳞状细胞癌中心体扩增中的作用及意义  

The p53-p21^waf1 pathway and centrosome amplification in oral squamous cell carcinomas

作　　者：蔡扬[1] 柳咏发[2] 杨宏 卢虹[1] 

机构地区：[1]贵阳医学院口腔医学系,贵州贵阳550004 [2]武警贵州总队医院口腔诊疗中心 [3]贵阳市口腔医院口腔内科

出　　处：《中华口腔医学杂志》2009年第6期332-335,共4页Chinese Journal of Stomatology

基　　金：国家自然科学基金（30460139）;教育部留学回国人员科研启动基金（N2005-1）.

摘　　要：目的了解口腔鳞状细胞癌（oral squamous cell cancer，OSCC）p21^waf1蛋白表达与中心体扩增的相关性，探讨p53-p21^waf1通路在OSCC中心体扩增中的作用及意义。方法8例正常口腔黏膜及27例OSCC石蜡包埋组织，采用间接免疫荧光双重染色法了解OSCC组织中心体扩增情况；采用流式细胞术及免疫组织化学方法对相应组织p21^waf1蛋白表达量及突变型p53蛋白进行检测，分析三者间的相关性。结果中心体数目扩增（〉2个／细胞）可见于78％（21／27）的OSCC组织，p21^waf1蛋白表达量在有中心体扩增的OSCC组织中[（0.878±0.081）]低于无中心体扩增的OSCC组织[（0.952±0.018），t=3.838，P=0.001]，OSCC组织中心体扩增程度与p21^waf1蛋白表达量间存在负相关关系（r=-0.472，P〈0.05）；p21^waf1蛋白表达量在OSCC组织p53阳性组[（0.823±0.071）]低于p53阴性组[（0.909±0.075），t=3.905，P〈0.01]，两者间存在负相关关系（r=-0.491，P〈0.05）。结论p53-p21^waf1通路可能参与了OSCC中心体循环调控，p53突变导致的p21^waf1蛋白表达下调在OSCC中心体扩增中可能起一定作用。Objective amplification in oral squamous To elucidate the possible role of cell carcinoma（OSCC）. Methods p53-p21^waf1 pathway for centrosome Formalin-fixed, paraffin-embedded tissues of 8 cases of normal oral epithelium tissues and 27 cases of OSCC tissues were examined for the expression of p21^waf1 and mutated p53 proteins by flowcytometry and immunohistochemistry, and centrosome status was investigated by indirect immunofluorescence double staining with antibodies to eentrosome protein gamma-tubulin and cytokeratin. The correlation between p21^waf1 , p53 and eentrosome amplification in OSCC was statistically analyzed by SPSS 12.0. Results All normal oral epithelium tissues showed normal centrosomes（ 1-2 centrosomes per cell） in epithelium cells, while 21 out of 27 cases（78% ） of OSCC showed the evidence of eentrosome amplification characterized by supernumerary centrosomes （ 〉 2 eentrosomes per cell） in a fraction of tumor cells. The quantity of p21^waf1 protein was lower in OSCC with centrosome amplification [（0.878±0.081）] than that in OSCC without centrosome amplification [（0.952±0.018）, t=3.838,P 〈0.011, and negative correlations were found between the quantity of p21^waf1 protein and the degree of centrosome amplification （r=-0.472, P〈0.05） , as well as the positive staining of p53 （r=-0.491 ,P〈0.01）. Conclusions p53-p21^waf1 pathway might involve in eentrosome duplication cycle in OSCC. Down-regulated p21wan protein, via p53 transactivation-dependent mechanism, was likely a contributing factor towards centrosome amplification in OSCC.

关 键 词：癌 鳞状细胞 中心体 流式细胞术 P21^WAF1 

分 类 号：R686[医药卫生—骨科学]