仙灵骨葆对卵巢切除大鼠股骨骨折愈合影响的实验研究  

EFFECT OF XIANLINGGUBAO ON FEMORAL FRACTURE HEALING IN OVARIECTOMIZED RATS

作　　者：穆树林[1] 张柳[1] 李震[1] 李强[1] 程潭[1] 田发明[1] 刘永强[1] 

机构地区：[1]华北煤炭医学院附属医院骨外科,河北唐山063000

出　　处：《中国修复重建外科杂志》2009年第6期690-693,共4页Chinese Journal of Reparative and Reconstructive Surgery

摘　　要：目的研究仙灵骨葆对卵巢切除大鼠股骨骨折愈合的作用。方法健康12周龄雌性SD大鼠40只,体重(258±14)g,随机分为4组,每组10只。A组仅作切口暴露腹腔;B组仅切除卵巢;C、D组切除卵巢同时制备右侧股骨中段横型骨折,髓内针固定,术后分别采用生理盐水及250mg/(kg·d)仙灵骨葆灌胃。于术后即刻、1、2、3、4及5周测量A、B组大鼠体重。术后5周取各组右侧股骨标本,双能X线骨密度仪测量股骨全长骨密度(total femur bone mineral density,tBMD)、远1/3段骨密度(distal femur bone mineral density,dBMD)和中1/3段骨密度(middle femur bone mineral density,mBMD),并行C、D组CR摄片、HE染色和免疫组织化学染色。结果B组大鼠体重从第3周开始显著高于A组(P<0.05),绝经后骨质疏松模型制备成功。CR摄片示D组骨痂量相对较多,骨折线模糊;C组骨痂量较少,骨折线清晰。B组tBMD和dBMD明显低于A组,D组mBMD明显高于C组,差异均有统计学意义(P<0.05);D组tBMD和dBMD较C组有增高趋势,但差异无统计学意义(P>0.05)。组织学观察D组与C组比较,骨折端大部分骨性愈合,骨髓腔可见较多毛细血管植入。免疫组织化学染色示C、D组BMP-2的积分吸光度(IA)值分别为2.2366±0.1818和3.7273±0.8742,VEGF的IA值分别为2.8355±0.5370和3.8396±0.2230,差异均有统计学意义(P<0.05)。结论仙灵骨葆可促进卵巢切除大鼠股骨骨折愈合,加快编织骨向板层骨的转化,这可能与上调BMP-2和VEGF表达相关。Objective To evaluate the effects of XiangLingGuBao （XLGB） on femoral fracture healing in ovariectomized （OVX） rats. Methods Forty 12-week-old female SD rats weighing （258±14）g were divided randomly into 4 groups （n=10 per group）： group A, sham operation by opening the abdominal cavity; group B, bilateral ovariectomy; group C, bilateral ovariectomy, transverse midshaft fracture of the right femur with intramedullary nail fixation, and normal saline by gavage; group D, bilateral ovariectomy, transverse midshaft fracture of the right femur with intramedullary nail fixation, and 250 mg/（kg·d） XLGB by gavage. The weight of rabbits in groups A and B was measured 0, 1, 2, 3, 4 and 5 weeks after operation. The right femur of each rat was obtained 5 weeks after operation. Total femur bone mineral density （tBMD）, distal femur bone mineral density （dBMD） and middle femur bone mineral density （mBMD） were measured by double energy X-ray absorptiometry CR filming, HE staining and immunohistochemistry staining of groups C and D were performed. Results The weight of rats in group B was obviously higher than that of group A at 3, 4 and 5 weeks after operation （P 〈 0.05）, indicating the animal model of postmenopausal osteoporosis was established successfully. CR films showed more callus and obscure fracture 1 ine in group D, while less callus and distinct fracture 1 ine in group C. The tBMD and the dBMD of group B were far less than that of group A, the mBMD of group D was significantly higher than that of group C （P 〈0.05）, the tBMD and the dBMD of group D were higher than that of group C, but no significant difference was evident between two groups （P 〉 0.05）. Histology observation showed, when compared with group C, most fracture ends in group D reached bone union, and the introduction of capillaries was evident in the marrow cavity. Immunohistochemistry staining demonstrated that the BMP-2 integrated absorbance （IA） value in groups C and D was 2.2366±0.1818 and 3.7273

关 键 词：骨质疏松症 骨折愈合 仙灵骨葆 大鼠 

分 类 号：R285.5[医药卫生—中药学]