Dicer在胃癌细胞和胃癌组织的表达及意义  

The expression and significance of Dicer in gastric cell lines and gastric cancer tissue

作　　者：聂娜[1] 吴小翎[1] 刘波[1] 

机构地区：[1]重庆医科大学附属第二医院消化内科,重庆400010

出　　处：《重庆医科大学学报》2009年第6期668-671,共4页Journal of Chongqing Medical University

摘　　要：目的:探讨Dicer在胃癌(Gastric carcinoma)细胞和胃癌组织中的表达及意义。方法:应用Western-Blot和逆转录-聚合酶链反应(Reverse transcriptase polymerase chain reaction,RT-PCR)检测正常胃粘膜细胞株GES-1、人胃癌细胞株SGC-7901,25例胃癌手术标本及癌旁配对组织Dicer的含量。结果:1.Western-Blot和RT-PCR结果表明Dicer在人胃癌细胞株SGC-7901的表达水平明显低于正常胃粘膜细胞株GES-1(P<0.05),在胃癌手术标本组织的表达水平明显低于癌旁配对组织(P<0.05);2.Dicer表达水平与胃癌分化程度有关。低分化组低于中分化组(P<0.05),中分化组低于高分化组(P<0.05)。结论:在胃癌细胞株和胃癌组织中Dicer表达明显降低并且Dicer表达与胃癌分化程度相关。Objective： To detect the expression and significance of Dicer in gastric cell lines and gastric cancer tissue. Methods： Western-Blot and reverse transcriptase polymerase chain reaction （RT-PCR） were used to detect the expression of Dicer in normal gastric mucosa cell line GES- 1, human gastric cancer cell line SGC-7901,25 surgical removed gastric cancer tissue and paired para-tumor tissue. Results： 1.The expression of Dicer in human gastric cancer cell line SGC-7901 was lower than that in normal gastric mucosa cell line GES-1 （P〈0.05） ,the expression of Dicer in gastric cancer tissue was lower than that in complete sequence tissue （P〈0.05）;2.The expression of Dicer is correlated with differentiation degrees of gastric cancer.The expression of Dicer in poorly differentiation group is lower than that in moderately differentiation group（P〈0.05）, and the expression of Dicer in moderately differentiation group is lower than that in well differentiation group （P〈0.05）. Conclusion： The expression of Dicer is obvious descend in gastric cancer tissue and gastric cancer cell lines and correlated with differentiation degrees of gastric cancer.

关 键 词：DICER 胃癌 逆转录-聚合酶链反应 

分 类 号：R5[医药卫生—内科学]