[^14C]苯并[a]芘在SD大鼠纹状体的动态分布研究  

Study on the dynamic distribution of [^(14)C]benzo(a)pyrene in the corpus striatum

作　　者：袁海艳[1] 汤艳[2] 程淑群[1] 涂白杰[1] 

机构地区：[1]重庆医科大学公共卫生学院劳动卫生与环境卫生教研室,重庆400016 [2]泸州医学院公共卫生系劳动卫生教研室,四川泸州460000

出　　处：《重庆医科大学学报》2009年第6期677-679,共3页Journal of Chongqing Medical University

基　　金：国家自然科学基金资助项目(NO.30671744)

摘　　要：目的:用r测量方法和光镜放射自显影研究[14C]苯并[a]芘([14C]Benzo(a)pyrene,[14C]BaP)在大鼠纹状体的动态分布,探讨BaP的神经毒性及其作用机制。方法:100只SD大鼠随机分为对照组和实验组,实验组尾静脉注射[14C]BaP3.7×105Bq/kg,对照组注射相同剂量的生理盐水。每组按照染毒后处死时间的不同,又分为1h、1d、2d、3d、7d共5个时间点,观察大鼠一般情况以及测定脏器系数,采用r测量方法和光镜放射自显影法观察[14C]BaP在纹状体的分布及其银粒密度。结果:BaP急性染毒后大鼠出现了一般情况的改变和脏器系数下降。r测量结果:纹状体每克脑组织含放射性占注射剂量的百分比(%ID/g)在第1d和第2d明显高于海马、大脑皮层(P<0.05);放射自显影结果:纹状体银粒在第1d达高峰,第2d显著减少,染毒后第7d仍可检测到银粒。结论:BaP能透过血脑屏障到达脑组织,主要分布和贮存于纹状体,具有一定的中枢神经毒性。Objective： To study the dynamic distribution of BaP in the corpus striatum by r-counting and light microscopic autoradiography,then to explore the neurotoxic mechanism of BaP. Methods： 100 SD male rats were divided into control group （n=40）and test group （n=60）, experimental animals were given a single intravenous injection of 3.7×10^5 Bq/kg of[^14C]BaP while the same doses of Normal Saline were given to the control group. The rats were sacrificed at 1h, 1 d, 2 d, 3 d and 7 d after the administration of radiolabelled BaP. During the experiment, some toxicological symptoms were observed and the ratios of brain-weight/body-weight were detected. Light micro- scopic autoradiography and r-counting were used to observe the dynamic distribution of BaP in the corpus striatum. Results： The change of toxicological symptoms are observed and the decrease ratios of brain-weight/body-weight are detected. R-counting shows that the percentage dose/g of [^14C]BaP in striatum is significant higher than in hippocampus and cortex at ld and 2 d after administration. Light micro-scopic autoradiography shows that the silver granules in striatum reach the peak in 1 d and sharp decrease in 2 d, which can be found even at 7d. Conclusion： BaP can penetrate the blood-brain barrier and distribute in corpus striatum, inducing CNS toxicity in SD rat.

关 键 词：苯并[A]芘 光镜放射自显影 r测量法 纹状体 

分 类 号：R135.1[医药卫生—劳动卫生]