粒-巨细胞集落刺激因子对单核细胞表达CCR2的影响  

Effects of granulocyte-macrophage colony-stimulating factor on the expression of cysteine-cysteine chemokine receptor 2 in THP-1 monocytes

作　　者：李炯[1] 罗文军[1] 陈以宽[1] 

机构地区：[1]重庆医科大学附属第二医院血管外科,重庆400010

出　　处：《重庆医科大学学报》2009年第6期706-709,共4页Journal of Chongqing Medical University

摘　　要：目的:探讨粒-巨细胞集落刺激因子(Granulocyte-macrophage colony-stimulating factor,GM-CSF)对体外培养的人单核细胞株THP-1表达半胱氨酸-半胱氨酸趋化因子受体2(Cysteine-cysteine chemokine receptor2,CCR2)mRNA和蛋白的影响。方法:以不同浓度的GM-CSF处理THP-1细胞,应用半定量RT-PCR方法检测CCR2mRNA表达,并应用Western蛋白印迹法进一步检测CCR2蛋白的表达。结果:对照组THP-1细胞表达较低水平的CCR2mRNA和蛋白,经GM-CSF刺激后各实验组CCR2mRNA和蛋白表达随GM-CSF浓度和时间的增加而增强。结论:GM-CSF可以刺激THP-1细胞CCR2mRNA和蛋白的表达,且其增加程度与GM-CSF刺激浓度及刺激时间呈正性相关。Objective： To investigate the effects of granulocyte-macrophage colony-stimulating factor on expressions ofCC chemokine receptor 2 （CCR2） mRNA and protein in cultured human THP-1 cell lines. Methods： THP-1 cell was stimulated by GM-CSF of different concentrations and time intervals.The mRNA expression of CCR2 was tested by RT-PCR, The protein expression of CCR2 was analyzed by Western blotting. Results： RT-PCR and Western blotting showed that THP-1 cell in control group expressed a low-level of CCR2 mRNA and protein. After stimulated by GM-CSF, the expression of CCR2 mRNA and protein increased in a concentration-and time-dependent manner. Conclusion： GM-CSF could stimulate THP-1 cell to express CCR2 mRNA and protein,in an concentration- and time-depen-dent manner.

关 键 词：粒-巨细胞集落刺激因子 单核细胞 CC趋化因子受体2 

分 类 号：R654.4[医药卫生—外科学]