高效液相色谱-串联质谱法测定妊娠肝内胆汁淤积患者血清牛磺结合型胆汁酸  

Simultaneous determination of serum tauro-conjugated bile acids in intrahepatic cholestasis of pregnancy patients by high performance liquid chromatography-tendem mass spectrometry

作　　者：邓文平[1] 王文娟[1] 邵勇[2] 邓丹[3] 丁敏[1] 

机构地区：[1]重庆医科大学医学检验系临床检验诊断学教育部重点实验室 [2]重庆医科大学附属第一医院妇产科 [3]重庆医科大学公共卫生学院卫生统计教研室,重庆400016

出　　处：《重庆医科大学学报》2009年第6期732-734,共3页Journal of Chongqing Medical University

基　　金：国家自然科学基金资助项目(20475069)

摘　　要：目的:研究妊娠肝内胆汁淤积症(Intrahepatic cholestasis of pregnancy,ICP)患者血清中牛磺结合型胆汁酸的变化。方法:收集ICP及ICP伴糖尿病、ICP伴病毒性肝炎患者和正常孕妇血清,经固相萃取,采用高效液相色谱-串联质谱(Highperfor-mance liquid chromatography-tendem mass spectrometry,HPLC-MS/MS)法测定牛磺结合型胆汁酸的含量。结果:ICP及ICP伴糖尿病、ICP伴病毒性肝炎患者血清中牛磺胆酸(Taurocholicacid,TCA)、牛磺鹅脱氧胆酸(Taurochenodeoxycholic acid,TCDCA)、牛磺脱氧胆酸(Taurodeoxycholic acid,TDCA)分别比正常孕妇有10倍、5倍和3倍以上的显著性增高(P<0.01)。ICP及ICP伴糖尿病、ICP伴病毒性肝炎三组间TCA和TCDCA的差异均有显著性(P<0.05)。结论:ICP患者有特征的血清牛磺结合型胆汁酸变化。Objective： To investigate the individual serum tauro-conjugated bile acid concentration in patients with Intrahepatic Cholestasis of Pregnancy （ICP） by high performance liquid chromatography-tendem mass spectro-metry （HPLC-MS/MS）. Methods： Bile acids were extracted from serum samples of ICP, ICP with Diabetes Mellitus, ICP with Virus Hepatitis patients and normal pregnant women. Serum tauro-conjugated bile acids were measured by HPLC-MS/MS. Results： The serum levels of taurocholic acid（TCA ）, taurochenode-oxycholic acid （TCDCA） and taurodeoxycholic acid （TDCA） in the three groups of ICP, ICP with Diabetes Mellitus, and ICP with Virus Hepatitis were significantly higher than those of the control group （P〈0.01）, and the differences and were 15-fold, 5-fold, 3-fold, respec-tively. The serum levels of TCA and TCDCA were statistically different among each two of patients of ICP, ICP with Diabetes Mellitus, ICP with Virus Hepatitis respectively （P〈0.05）. Conclusion： The concentrations of individual tauro-conjugated bile acids have specific features in ICP patients.

关 键 词：胆汁酸 血清 妊娠肝内胆汁淤积症 高效液相色谱 串联质谱 

分 类 号：R714.255[医药卫生—妇产科学]