丹参酮Ⅱ-A抑制血管合成醛固酮及醛固酮合酶基因CYP11B2信使核糖核酸表达  

Tanshinone ⅡA Inhibits Aldosterone Production and CYP11B2 mRNA Expression in Blood Vessels

作　　者：吴平生[1] 梁欣伟[1] 郭志刚[1] 张远慧[1] 刘伊丽[1] 聂道海 刘品明 郭庆秋 张榕华 赖文岩[1] 

机构地区：[1]中国人民解放军第一军医大学附属南方医院

出　　处：《中国循环杂志》1998年第3期175-177,共3页Chinese Circulation Journal

摘　　要：目的：说明丹参酮ⅡＡ对血管合成血管紧张素Ⅱ及醛固酮的作用。方法：用大鼠肠系膜动脉离体灌注、高效液相色谱法和放射免疫法检测肠系膜动脉中合成的血管紧张素Ⅱ及醛固酮。用逆转录聚合酶链反应（ＲＴ－ＰＣＲ）及ＳｏｕｔｈｅｒｎＢｌｏｔ法检测血管中醛固酮合酶基因ＣＹＰ１１Ｂ２信使核糖核酸（ｍＲＮＡ）的表达情况。结果：丹参酮ⅡＡ抑制血管合成醛固酮，但不能抑制血管合成血管紧张素Ⅱ。丹参酮ⅡＡ抑制血管中醛固酮合酶基因ＣＹＰ１１Ｂ２ｍＲＮＡ的表达。结论：丹参酮ⅡＡ治疗心血管疾病的机制可能与其抑制血管合成醛固酮有关。Objective:To find the pharmacological effects of tanshinone ⅡA on angiotensin Ⅱ and aldosterone production in blood vessels. Methods:Using ex vivo rat mesenteric artery perfusion,HPLC,and RIA to measure the amount of angiotensin Ⅱ and aldosterone produced in vascular tissue,and by RT PCR and Southern Blot to detect the expression of aldosterone synthase gene—CYP11B2 mRNA in aorta. Results:The production of aldosterone but not of angiotensin in mesenteric artery was inhibited ( p <0.05) and the CYP11B2 mRNA expression in aorta was down regulated by tanshinone ⅡA. Conclusion:The inhibition of vascular aldosterone by tanshinone ⅡA might contribute to its therapeutic effect on cardiovascular diseases.

关 键 词：丹参酮ⅡA 醛固酮 醛固酮合酶基因 MRNA 中药 

分 类 号：R285.5[医药卫生—中药学]