Chemical composition and antimicrobial activity of essential oil isolated from the cultured mycelia of Ganoderma japonicum  

作　　者：Dandan Liu Zheng Hu Zhigang Liu Bo Yang Wenjuan Tu Liang Li 

机构地区：[1]The Key Laboratory of Industrial Microbiology of Hubei Province, Hubei University of Technology, Wuhan 430068, China [2]School of Biochemistry and Molecular Biology of Australian National University, Canberra, Australia 0200

出　　处：《Journal of Nanjing Medical University》2009年第3期168-172,共5页南京医科大学学报（英文版）

基　　金：supported by the Natural Science Foundation of Hubei Province(2004ABA228)

摘　　要：Objective：To explore a new natural antibiotic. Methods：The chemical composition of the essential oil from Ganodermajaponicum （G. japonicum）mycelia was analyzed by gas chromatography-mass spectrometry（GC-MS）, The antimicrobial activity of the oil was evaluated against eighteen microorganisms, including bacteria, mildew and yeast by using a disc diffusion method. Furthermore, the minimum inhibitory concentrations（MIC） and the minimum bactericidal concentrations（MBC） of the essential oil against twelve clinical pathogens were determined. Results：The main components of the oil were nerolidol, decadienal, linalool and benzyl alcohol. The antimicrobial results indicated that the oil inhibited all the tested bacterium, especially Methicillin-resistant Staphylococcus aureus （MRSA） in which the antibacterial activity exhibited a MBC of 1.03 mg/ml. Conclusion： The essential oil of G. japonicum mycelium has significant inhibitory activity. It is a potential medicinal resource that can be used as a natural antibiotic.Objective：To explore a new natural antibiotic. Methods：The chemical composition of the essential oil from Ganodermajaponicum （G. japonicum）mycelia was analyzed by gas chromatography-mass spectrometry（GC-MS）, The antimicrobial activity of the oil was evaluated against eighteen microorganisms, including bacteria, mildew and yeast by using a disc diffusion method. Furthermore, the minimum inhibitory concentrations（MIC） and the minimum bactericidal concentrations（MBC） of the essential oil against twelve clinical pathogens were determined. Results：The main components of the oil were nerolidol, decadienal, linalool and benzyl alcohol. The antimicrobial results indicated that the oil inhibited all the tested bacterium, especially Methicillin-resistant Staphylococcus aureus （MRSA） in which the antibacterial activity exhibited a MBC of 1.03 mg/ml. Conclusion： The essential oil of G. japonicum mycelium has significant inhibitory activity. It is a potential medicinal resource that can be used as a natural antibiotic.

关 键 词：Ganodermajaponicum essential oil antimicrobial activity clinical pathogens 

分 类 号：R978.1[医药卫生—药品] R284.1[医药卫生—药学]