精子因素对精子载体法制备转基因山羊的影响  

Effect of Sperm Parameters on Exogenous DNA Binding to Goat Sperm

作　　者：赵永聚[1,2] 

机构地区：[1]西南大学动物科技学院重庆市牧草与草食家畜重点实验室,重庆北碚400716 [2]第三军医大学基础部实验动物学教研室,重庆400038

出　　处：《动物学杂志》2009年第3期141-145,共5页Chinese Journal of Zoology

基　　金：国家自然科学基金项目(No.30600430);国家科技重大专项项目(No.2008ZX08008-004);西南大学博士后启动基金项目

摘　　要：精子具有主动结合、转运、整合外源DNA的能力,并在受精时导入卵母细胞,获得转基因动物。精子介导基因转移(sperm-mediated gene transfer,SMGT)是目前获得转基因动物简单而高效的方法之一。精子因素是影响SMGT方法生产转基因动物的重要方面。本论文结合我们的研究针对转染用山羊(Caprahircus)精液的来源、精子质膜完整性、精液品质及发育阶段等精子因素影响精子结合外源DNA和SMGT方法生产转基因山羊的效率进行了论述,并从这些影响因素入手,提出了筛选精子供体、保持精液品质、调控质膜等措施,提高精子转染外源DNA能力和生产转基因动物的效率。Sperm-mediated gene transfer （SMGT） appears to be a simple, efficient, and inexpensive method for genome modification of animals. DNA uptake by the mammalian spermatozoa is a very specific and highly regulated phenomenon. The sperm parameters, such as sperm donor, plasma membrane, sperm quality, and the treatment methods affect not only the ability and efficiency of sperm to pick up exogenous DNA and to internalize the DNA into sperm nuclei, but also the vector type and transfection method used. The efficiency of picking up exogenous DNA by goat spermatozoa decreases with age. Different goat breeds exhibit different abilities in binding foreign genes. Selection of sperm donors and optimization of DNA uptake are the key steps for the successful outcome of SMGT. The use of frozen-thawed spermatozoa is an efficient method for production of transgenic offspring.

关 键 词：山羊 外源DNA 精子 供体 精子载体法 

分 类 号：Q78[生物学—分子生物学] S814.8[农业科学—畜牧学]