多溴联苯醚对鲫鱼离体肝脏组织中CAT和GSH-Px的影响  

The in vitro effects of tetrabromodiphenyl ether and decabromodiphenyl ether on the activities of catalase and glutathione peroxidase in the liver of Carassius auratus.

作　　者：吴伟[1,2] 聂凤琴[2] 瞿建宏[1] 

机构地区：[1]中国水产科学研究院淡水渔业研究中心//中国水产科学研究院内陆渔业生态环境与资源重点开放实验室,江苏无锡214081 [2]南京农业大学渔业学院,江苏无锡214081

出　　处：《生态环境学报》2009年第2期408-413,共6页Ecology and Environmental Sciences

基　　金：中央级公益性科研院所基本科研业务费专项资金项目(2007JBFB02);中国水产科学研究院内陆渔业生态环境与资源重点开放实验室开放课题(YM2007-10)

摘　　要：以鲫鱼(Carassiusauratus)为试验材料,研究了在离体条件下经不同质量浓度的2,2’,4,4’-四溴联苯醚(BDE-47)和2,2',3,3',4,4',5,5',6,6'-十溴联苯醚(BDE-209)暴露后,鲫鱼肝脏组织中过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)活性的动态变化。结果表明,采用质量浓度为0.10～10.00mg·L-1的BDE-47和5.6～100.00mg·L-1的BDE-209分别处理鲫鱼肝脏组织30min,0.10mg·L-1BDE-47和5.6mg·L-1BDE-209试验组鲫鱼肝脏组织中CAT和GSH-Px活性与对照组相比无显著差异(P>0.05),其余各试验组的CAT和GSH-Px活性随BDE-47及BDE-209质量浓度的增加而逐渐下降,均与BDE-47及BDE-209的质量浓度呈明显的相关关系(P<0.01)。这说明BDE-47和BDE-209对鲫鱼肝脏产生了氧化损伤,具有生化毒性影响。The objective of this in vitro study was to evaluate the toxic effects of 2,2＇,4,4＇,-tetrabromodiphenyl ether （PBDE-47） and 2,2＇,3,3＇,4,4＇,5,5＇,6,6＇-decabromodiphenyl ether （PBDE-209） on the activities of catalase （CAT） and glutathione peroxidase （GSH-Px） in the liver of Carassius auratus. Activities of CAT and GSH-Px were measured after a 30 min exposure at 25 ℃ to PBDE-47 and PBDE-209 quality concentration 0.10-10.00 mg·L^-1 and 5.6-100.00 mg·L^-1, respectively. No significant difference （P〉0.05） was found between the controls and testing groups exposed to 0.10 mg·L^-1PBDE-47 and 5.6 mg·L^-1PBDE-209. In contrast, a very significant inhibition （P〈0.01） on the activities of CAT and GSH-Px was observed in the remaining test groups, especially those exposed to 〉0.10 mg·L^-1 PBDE-47 and 5.6 mg·L^-1 PBDE-209, showing an obvious concentration-depended negative correlation with both PBDE congeners. These results clearly indicate that PBDE-47 and PBDE-209 can cause hepatic oxidative stress and toxicity to Carassius auratus.

关 键 词：2  2’  4  4’-四溴联苯醚 2  2’  3  3’  4  4’  5  5’  6  6’-十溴联苯醚 鲫鱼 肝脏 离体 氧化胁迫 

分 类 号：X503.22[环境科学与工程—环境工程]