抗颤宁方对帕金森病大鼠行为学及脂质过氧化的影响  

Effect of Kangchanning on neurobehavior and lipid peroxidation of PD rat

作　　者：张希廉[1] 田军彪[1,2] 耿百乐[1] 石玫[3] 顾平[4] 

机构地区：[1]河北医科大学中医院 [2]河北省中医院科教科,河北石家庄050011 [3]河北省石家庄市中医院,河北石家庄050051 [4]河北省脑老化与认知神经科学实验室,河北石家庄050031

出　　处：《河北中医》2009年第3期441-443,共3页Hebei Journal of Traditional Chinese Medicine

基　　金：河北省中医药管理局2008年度中医药;中西医结合科研计划项目(编号:2008026);河北省科技支撑计划(编号:09276103D-6)

摘　　要：目的探讨抗颤宁方对帕金森病(parkinsong disease,PD)大鼠行为学及脂质过氧化的影响。方法将60只健康SD雌性大鼠,随机分出8只为假手术组,右侧黑质注射0.9%氯化钠注射液;其余为造模组,右侧黑质注射6-羟基多巴胺(6-OHDA),将造模成功大鼠随机分为模型对照组、中药组和西药组,各8只。于术后次日开始给药,中药组给予抗颤宁方33.5 mg/kg,西药组给予美多巴125 mg/kg,模型对照组与假手术组给予等体积的蒸馏水,均灌胃14 d。检测各组旋转行为,并记录旋转开始时间、结束时间和旋转次数;用比色法分别测定血清中超氧化物歧化酶(SOD)活性及丙二醛(MDA)、一氧化氮(NO)含量。结果中药组、西药组给药后平均转速、持续时间均下降,与本组治疗前比较差异均有统计学意义(P<0.05),说明中药组、西药组均可改善PD大鼠的临床症状;且中药组给药后平均转速、持续时间均低于西药组给药后(P<0.01),说明中药组在改善PD大鼠部分症状方面优于西药组。模型对照组、西药组SOD活性均明显下降,与假手术组比较差异均有统计学意义(P<0.01),中药组SOD活性与假手术组比较差异无统计学意义(P>0.05);模型对照组MDA、NO含量明显升高,与假手术组、中药组、西药组比较差异均有统计学意义(P<0.05),中药组、与西药组MDA含量与假手术组比较差异无统计学意义(P>0.05)。结论抗颤宁方具有改善脑组织氧化应激状态及对抗NO的神经毒性等作用。Objective To study the effect of Kangchanning on neurobehavior and lipid peroxidation of PD rat.Methods 60 SD rats were regarded as experimental aninmls. 8 rats of them was sham operation group, which were injected by 0.9% NaC1 solution at RSN. Other animal were injected by 6 - OHDA at RSN for establishment of Parkinson disease （PD）. Model rats were randomly divided into model control group, traditional medicine group and western medicine group. 8 rats were in each group. Rat in traditional medicine group were treated by Kangchanning, 33.5 mg/kg. Rat in western medicine group were treated by madopar, 125 mg/kg. Rat in sham operation group and model control group were treated by isovolumie distilled water. Circling behavior, eireumrotation initial time, closing time and number of circle were recorded. SOD, MDA and NO in serum were measured by ehromatometry. Results The rotation speed and duration in traditional medicine group and western medicine group obviously decreased after treatment （ P 〈 0.05）. The rotation speed and duration in traditional medicine group were lower than those in western medicine group （ P 〈 0.01）. The activity of SOD in model group and western medicine group were decreased comparison with that in sham operation （ P 〈0.01）. There was no difference between traditional medicine group and sham operation （ P 〉 0.05）. The contents of NO and MDA in model control group were obviously increased （ P 〈 0.05）. Conclusion Kangchanning can impwve oxidative stress of brain tissue and antagonize neurotoxicity of NO.

关 键 词：帕金森病 超氧物歧化酶 丙二醛 一氧化氮 模型 动物 

分 类 号：R332[医药卫生—人体生理学] R745.7[医药卫生—基础医学]