HPLC法测定益骨胶囊中淫羊藿苷的含量  

Determination the content of icariin in Yigu Capsules by HPLC

作　　者：王冰[1] 唐振香 郭瑞臣[2] 

机构地区：[1]威海市立医院,山东威海264200 [2]山东大学齐鲁医院,山东济南250012

出　　处：《齐鲁药事》2009年第5期271-272,共2页qilu pharmaceutical affairs

摘　　要：目的建立测定益骨胶囊中淫羊藿苷含量的方法.方法采用高效液相色谱法,色谱柱为Venusil XBP-C18柱(250mm×4.6mm,5μm),流动相为乙腈-水(30∶70,V/V),流速1.0mL·min-1,检测波长270nm.结果淫羊藿苷与杂质峰的分离度符合要求,淫羊藿苷在0.25～8μg·mL-1范围内峰面积与进样量线性关系良好,r=0.99349(n=5).平均加样回收率为96.07%,RSD为4.46%(n=5).结论本文建立的分析方法简便、重现性好,可用于益骨胶囊中淫羊藿苷含量测定.OBJECTIVE To establish a method for the determination of icariin in Yigu Capsules. METHODS Venusil XBP-C18 column （250min×4.6mm,5μm） was used;water-acetonitrile （70：30, V/V ） were used as mobile phase, the flow rate was 1.0mL·min^-1;The detection wavelength was 270nm;RESULTS The line correlation was observed from 0.25-8μg·mL^-1 ,the averagere recoveries were 96.07%, RSD was 4.46% （n = 5）. CONCLUSION The method was simple, accurate and reproducible.

关 键 词：淫羊藿苷 益骨胶囊 含量测定 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]