不同组成比例的七叶皂苷钠抗炎作用及急性毒性研究  

Study on anti-inflammatory effects and acute toxicity of sodium aescinate with different proportion

作　　者：薛云丽 侯金玲[2] 王芃[2] 张秀芹[2] 李杰[2] 

机构地区：[1]山东绿叶制药有限公司,山东烟台264003 [2]山东省医学科学院药物研究所药理室,山东济南250062

出　　处：《齐鲁药事》2009年第5期305-307,共3页qilu pharmaceutical affairs

摘　　要：目的观察并比较不同比例的七叶皂苷钠样品的抗炎及毒性作用,筛选确定纯化分离七叶皂苷钠的最佳活性部位,为优化精制工艺提供参考.方法采用耳肿胀及腹腔毛细血管通透性2种炎症模型,观察四个具有七叶皂苷钠A、B、C、D不同比例样品的抗炎作用,并测定静脉给药的LD50.结果所研究的七叶皂苷钠四个样品腹腔注射给药能明显抑制二甲苯所致小鼠耳肿胀,皮下注射给药均能明显抑制醋酸所致小鼠腹腔毛细血管通透性的增加.四个样品的LD50分别为9811199.76mg·kg-1、9811123.43mg·kg-1、9811164.14mg·kg-1、9803159.57mg·kg-1.结论四个样品的抗炎作用以981119、981112和981116略好,毒性以981119和980315较低,因此样品981119所含七叶皂苷钠为最佳活性部位.OBJECTIVE To study and compare the anti-inflammatory effects and acute toxicity of sodium aescinate with different proportion of Aescin A,B,C and D, so as to establish the best produce technology of Sodium Aescinate. METHODS To observe the effects of sodium aescinate on the swelling index of ears of mice induced by dimethylbenzene and the permeability of capillary vessel by acetic acid. To measure the LD50 of the sodium aescinate. RESULTS Sodium Aescinate can significantly inhibit the swelling index of ears when given intraperitoneally and inhibit the permeability of capillary vessel when given subcu- taneouslly. The LD50 of the sodium sescinate samples of 981119, 981112,981116 and 980315 are 9.76mg·kg^-1, 3.43mg·kg^-1 ,4. 14mg·kg^-1 and 9.57mg· kg^-1 ,respectively. CONCLUSION The anti-inflammatory effects of sodium aescinate sam- ples of 981119,981116 and 981112 are better than 980315,and the toxicity of the samples of 981119 and 980315 are much low- er. The sample of 981119 is the best for the therapy of peripheral vascular with higher activity of anti-inflammatory and lower toxicity.

关 键 词：七叶皂苷钠 抗炎作用 毒性 

分 类 号：R285.5[医药卫生—中药学]