短蛸呼吸、循环、排泄和生殖系统甲硫氨酸脑啡肽的免疫组织化学定位  

Immunohistochemical localization of met-Enkephalin in the respiratory system,circulatory system,excretory system,reproductive system of Octopus ocellatus

作　　者：沙爱龙[1] 孙虎山[2] 王宜艳[2] 

机构地区：[1]新疆生产建设兵团塔里木畜牧科技重点实验室,塔里木大学动物科学学院,新疆阿拉尔843300 [2]鲁东大学生命科学学院,山东烟台264025

出　　处：《南方水产》2009年第3期59-62,共4页South China Fisheries Science

基　　金：国家重点基础研究发展计划(973计划)项目(G1999012005)

摘　　要：采用免疫组织化学SABC法对短蛸(Octopus ocellatus)呼吸、循环、排泄和生殖系统中甲硫氨酸脑啡肽(met-Enk)进行定位研究。结果表明,短蛸呼吸系统、循环系统、排泄系统和生殖系统中都有met-Enk存在,充分说明met-Enk在短蛸体内分布是比较广泛的。可以发现短蛸met-Enk大都阳性较弱。met-Enk可能参与短蛸呼吸、循环、泌尿、生殖和内分泌等功能的调节。而met-Enk在短蛸不同部位分布密度的不同,可能与各器官的功能不同有关。Met-Enkephalin in different tissues of Octopus ocellatus was observed and analyzed with immunohistochemical SABC technique. The results showed that met-Enkephalin existed in the respiratory system, circulatory system, excretory system, reproductive system, suggesting that distribution of met-Enkephalin was comprehensive in the O. ocellatus. It was found that most tissues presented weak met-Enkephalin immunoreaction positive in the O. ocellatus. Met-Enkephalin was likely to accommodate respiration, circulation, excretion, procreate, incretion of O. ocellatus. And the different density of met-Enkephalin might be referable to different function of organs.

关 键 词：短蛸 呼吸系统 循环系统 排泄系统 生殖系统 甲硫氨酸脑啡肽 免疫组织化学 

分 类 号：Q26[生物学—细胞生物学] Q959.216.1