用CBMN法评价饮用水处理流程中有机提取物的细胞毒性  

The Cytotoxicity Assessment of Organic Extracts from Drinking Water in Technological Process by Cytokinesis block Micronucleus Assay

作　　者：刘艳红[1] 李素文[1] 王文华[2] 柴文 金维荣 

机构地区：[1]北京师范大学生物系 [2]中国科学院生态环境研究中心 [3]北京市第九水厂

出　　处：《环境科学》1998年第3期42-45,共4页Environmental Science

基　　金：北京市自然科学基金

摘　　要：利用ＣＨＯ细胞胞质分裂阻断微核（ＣＢＭＮ）法，对某自来水厂７个处理工艺流程水中的有机提取物的细胞毒性进行了评价．在实验剂量范围内，比较了各样品的含微核的双核细胞率（ＢＮＭＮ）、核分裂指数（ＮＤＩ）和胞质分裂阻断增殖指数（ＣＢＰＩ）．实验表明，源水加氯后比源水的细胞毒性增大；经机加池和煤砂滤池处理后的水样毒性较大，其中煤砂滤池水毒性最大，机加池水次之；经炭吸附后的水样比炭吸附前水样的毒性下降；管网水的毒性较低．By means of the Cytokinesis block micronucleus(CBMN)assay in CHO cell,the cytotoxicity of organic extracts from drinking water was evaluated.The parameters,including binucleated cells with MN (BNMN),nuclear division index (NDI) and cytokinesis block proliferation index (CBPI),were compared in the range of doses tested for the individual extracts.The results indicated that the chloronated source water was more toxic than the source water.The water samples unfiltrated by carbon were more toxic than the filtrated ones.The sand filtration water was the most toxic,the flocculation water was less toxic than sand filtration water ,and the water was the least toxic among seven samples.

关 键 词：水处理工艺 有机提取物 细胞毒性 饮用水 消毒 

分 类 号：TU991.25[建筑科学—市政工程]