雌激素诱导小鼠肠道Cajal间质细胞内ERK快速活化的研究  

17-β estradiol induces rapid activation of extracellular signal-regulated kinase in interstitial cells of Cajal

作　　者：李凡[1] 黄颖[1] 童卫东[1] 刘宝华[1] 

机构地区：[1]第三军医大学第三附属医院野战外科研究所普通外科,重庆400042

出　　处：《重庆医学》2009年第11期1354-1355,1358,共3页Chongqing medicine

基　　金：重庆市自然科学基金资助项目(2006BB5114)

摘　　要：目的研究17-β雌二醇(E2)对小鼠肠道Cajal间质细胞(ICC)中细胞外信号调节激酶(ERK1/2)的活化作用。方法利用原代培养小鼠ICC,采用Western blot分析E2诱导ICC内ERK1/2磷酸化的变化。结果E2作用于ICC,5 min即能诱导ERK1/2磷酸化,15 min后磷酸化ERK1/2蛋白表达达到最高,并从此逐渐降低;雌激素受体拮抗剂ICI182780可以阻断E2活化ERK1/2的作用。结论E2可以通过雌激素膜受体诱导ICC内ERK1/2的快速活化。Objective To investigate the effect of 17-β estradiol（E2） on the activation of extracellular signal-regulated kinase （ERK1/2） in interstitial cells of Cajal（ICC）. Methods Western blot were performed to examine the phosphorylation of ERK1/2. Results E2 rapidly induced phosphorylation of ERK1/2 within 5rain, E2-induced phosphorylation of ERK1/2 reached its peak at 15rain and rapidly declined thereafter. The effect of E2 on ERK1/2 activation were abolished by a selective estrogen receptor antago- nist,ICI182780. Conclusion E2 can induce rapid activiation of ERK1/2 in ICC through membrane estradiol receptors（mER）.

关 键 词：雌激素膜受体 CAJAL间质细胞 雌激素 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]