细胞分子生物学技术在靶向药物筛选中的应用  

Applications of Cell and Molecular Biotechnology in Targeted Drug Screening

作　　者：刘明[1] 赵琦[1] 李亚男[1] 胡文祥[2] 

机构地区：[1]首都师范大学生命科学学院,北京100048 [2]首都师范大学物理有机与药物化学研究所,北京100048

出　　处：《生物技术通报》2009年第6期62-66,共5页Biotechnology Bulletin

基　　金：国家自然科学基金资助项目(20872095)

摘　　要：随着细胞及分子生物学的发展,新技术方法越来越多地用于新靶点建立和药物筛选研究,为药物设计、靶点的选择和用药方案的确定提供理论依据,同时使药物筛选有了更高的特异性,对药物筛选和药理学研究起到了极大的促进作用。论述了功能基因的筛选、高通量细胞筛选和高内涵筛选技术、反义核酸技术、转基因/基因敲除技术、基因芯片、蛋白质芯片、组织芯片、酶免疫分析、荧光免疫分析、流式细胞技术等方法在靶向药物筛选中的应用。With the development of molecular and cell biology, pharmacology, physiology, chemistry and pathology, growing biotechnologies were applied in the research of drug design and screening that has speeded up the rate of new drug development. This article summarized recent advances of molecular and cell techniques, such as expression profiling normal and diseased tissues , high- throughtput cell-based screening , high content screening, technology,gene chip, protein chip, tissue microarray, surface plasmon resouanc, enzymeimmuoassay, fluorescenceimmunoassay and flowcytometer, which could help to improve the pharmaceutical screening, pharmacology and receptorology research work to make great progress.

关 键 词：生物技术 药物筛选 分子药理学 受体学 

分 类 号：R96[医药卫生—药理学]