两种阳离子纳米基因载体及植物基因介导效果的研究  

Study on Cationic Nanoparticles as Gene Carriers and the Efficiency for Transferring Gene into Plant Cells

作　　者：宋瑜[1] 李颖[1] 崔海信[1] 李瑶[1] 

机构地区：[1]中国农业科学院农业环境与可持续发展研究所,北京100081

出　　处：《生物技术通报》2009年第6期75-80,共6页Biotechnology Bulletin

基　　金：国家"863"重大项目(2006AA10A203);国家博士后科学基金项目(20070420435)

摘　　要：以阳离子聚乙烯亚胺(polyethylenimine,PEI)和壳聚糖(chitosan,CS)作为两种植物基因载体,分别制备了载基因PEI纳米粒(PEI/DNA)和壳聚糖-DNA纳米粒(CS/DNA),并对其形态、粒度分布、包封率、DNA结合的稳定性及纳米颗粒对DNA的保护等方面进行表征。并以GFP基因为报告基因进行植物细胞转染,比较两者转化效率。结果表明PEI/DNA纳米粒稳定性,对DNA的保护以及转染效率等方面均优于壳聚糖-DNA纳米粒。Polyethyleneimine （PEI） and chitosan（CS） were used to prepare PEI-DNA （PEI/DNA） and CS-DNA （CS/DNA） complexes as gene carriers for DNA delivery. The characteristics of the complexes such as morphology, particle size, association efficiency, stability of DNA binding, and protection ability to DNA were investigated. Transfection efficiency mediated by PEI/DNA and CS/DNA were examined with GFP gene as report gene, respectively. The results showed that PEI/DNA nanoparticles were superior to chitosan-DNA nanoparticles in stability, protection ability of DNA and transfection efficiency Arabidopsis thaliana to protoplasts.

关 键 词：聚乙烯亚胺 壳聚糖 纳米粒 基因载体 转基因 

分 类 号：Q943.2[生物学—植物学]