贵州不同地区坚龙胆中龙胆苦苷含量测定  

Contents Determination of Gentiopicroside in Gentiana Rigescens from Different Habitas in Guizhou Province

作　　者：韩双[1] 李玮[1] 席先蓉[1] 

机构地区：[1]贵阳中医学院药学系,550002

出　　处：《医药导报》2009年第6期783-785,共3页Herald of Medicine

基　　金：教育部春晖计划基金资助项目(基金编号:Z2005-1-52001)

摘　　要：目的测定贵州产坚龙胆中龙胆苦苷的含量,并评价不同产地坚龙胆的质量。方法采用高效液相色谱,Alltima C18(4.6 mm×250 mm,5μm)色谱柱,以甲醇-水(30:70)为流动相;流速1.0 mL.min-1;检测波长为270 nm;柱温为30℃。利用正交实验以龙胆苦苷含量为指标研究了坚龙胆的最佳提取方法。结果龙胆苦苷在0.08～0.64 mg.mL-1(r=0.999 9)范围内与峰面积线性关系良好;平均回收率为102.4%,RSD为1.69%(n=5)。测定了11个产地坚龙胆中龙胆苦苷的含量在2.113%～4.808%间。结论贵州不同地区间龙胆质量均符合《中华人民共和国药典》2005年版要求,各地区之间无明显差异。Objective To determine the content of gentiopicroside and evaluate the qulity of Gentiana rigescens from different habitats in guizhou province. Methods HPLC was used with Alhima C lS （4.6 mm× 250 mm , 5 μm） , methanolwater（ 30 ： 70） as mobile phase, detector wavelength at 270 nm and column temperature at 30℃. The optimal extraction method for gentiopicroside was sdudied by orthogonal experiments. Results The calibration curve was linear with gentiopicroside in the range of 0.08 - 0.64 mg · mL^-1（ r = 0.999 9） , the average recovery was 102.4% with RSD of 1.69% （n = 5）. The contents of gentiopicroside in Gentiana rigescens from 11 habits were between 2. 113% -4. 808%. Conclusion The quality of Gentiana rigescens from different habitats of guizhou province are consistent with the chinese Pharmacopoeia 2005, and there is no notable difference among them.

关 键 词：坚龙胆 龙胆苦苷 提取工艺 

分 类 号：R282.71[医药卫生—中药学] R927.4[医药卫生—中医学]