1，2-萘醌-4-磺酸钠作为显色剂分光光度法测定阿魏酸哌嗪  

Spectrophotometric determination of piperazine ferulate using sodium 1,2-naphthoquinone-4-sulfonate as a chromogenic reagent

作　　者：吴伶俐[1,2] 魏献军[1] 高林晓[1] 李全民[1] 

机构地区：[1]河南师范大学化学与环境科学学院,河南省环境污染控制重点实验室,新乡453007 [2]河南广播电视大学农医学院,郑州450008

出　　处：《分析试验室》2009年第6期53-56,共4页Chinese Journal of Analysis Laboratory

基　　金：河南省科技厅自然科学基金(082300420210,082300420220)项目资助

摘　　要：建立了用1，2-萘醌-4-磺酸钠测定阿魏酸哌嗪的新方法。在pH13．0的KCl—NaOH缓冲溶液中，阿魏酸哌嗪与1，2-萘醌-4-磺酸钠反应形成红褐色产物，其最大吸收波长为484nm。阿魏酸哌嗪浓度在0．4～80μg／mL范围内呈现良好线性关系。线性回归方程为A=0．07742＋0．01216p（μg/mL），线性相关系数r=0．9986，表观摩尔吸光系数5．8×10^3 L／mol／cm，相对标准偏差（RSD）和检出限分别为0．84％和0．29μg／mL。对药物样品中阿魏酸哌嗪的含量进行测定，平均回收率在95．1％～103．3％。A rapid, simple and sensitive method for the determination of piperazine ferulate using sodium 1, 2-naphthoquine-4-sulfonate as a chromogenic reagent is established in this paper. It is based on the fact that a russety product can be formed by the reaction between piperazine ferulate and sodium 1, 2-naphthoquine-4-sulfonate in pH 13.0 buffer solution. Beer＇ s law is obeyed in a range of 0.4 - 80 μg/mL for piperazine ferulate at the maximum absorption of 484 nm （ ε = 5.8 × 10^3 L· mol· cm^-1）. The linear regression equation of the calibration curve is A = 0.07742 ＋ 0.01216p （μg/mL）, with a correlation coefficient of 0.9986. The detection limit is 0.29 μg/mL （3σ/k）, RSD is 0.84% and recoveries are from 95.1% to 103.3%. Furthermore, the proposed method has been successfully applied to the determination of piperazine ferulate in pharmaceutical samples.

关 键 词：阿魏酸哌嗪 1 2-萘醌-4-磺酸钠 分光光度法 

分 类 号：O657.3[理学—分析化学]