量子点标记链霉亲和素荧光免疫分析测定雌三醇  

Determination of estriol by fluorescence immunoassay with streptavidin conjugated quantum dots

作　　者：钱建瑞[1] 赵艳伟[1] 梁艳[1] 李立华[1] 王术皓[1] 

机构地区：[1]聊城大学化学化工学院,聊城252059

出　　处：《分析试验室》2009年第6期104-107,共4页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金(20877037);山东省自然科学基金(2005ZX12)项目资助

摘　　要：以生物素化羊抗兔免疫球蛋白和量子点标记链霉亲和素为荧光探针，采用竞争免疫分析模式，建立了一种灵敏度高、选择性好的检测雌三醇的荧光免疫分析方法。对几种测量参数如温育时间和温度、缓冲溶液pH、试剂浓度等进行了优化；方法的线性范围为0．01～1000ng/mL，检出限0．0029ng／mL，血清样品加标回收率在91％-117％之间。A sensitive and selective method for the determination of estriol by fluorescence immunoassay （FIA） was established on the basis of competing mode immunoassay. The avidin-biotin system as signal amplification system and QDs-avidin probe were applied to the method. After the conditions of immunoreaction such as the time and temperature of incubating, the pH of the buffer solution and the concentration of the reagents optimized, the linear range and the detection limit of the method were 0.01 - 1000 ng/mL, 2.9 - 10^-3 ng/mL. The recoveries are in the range of 91% - 117% in human serum.

关 键 词：量子点 荧光免疫分析 雌三醇 生物素-亲和素系统 

分 类 号：O657.32[理学—分析化学]