同时接种解磷细菌与丛枝菌根真菌对低磷土壤红三叶草养分利用的影响  

Effect of co-inoculation with arbuscular mycorrhizal fungi and four different phosphate-solubilizing bacteria on nutrients uptake of red clover in a low phosphorus soil

作　　者：秦芳玲[1] 田中民[2] 

机构地区：[1]西安石油大学化学化工学院,陕西西安710065 [2]西安交通大学生命科学院,陕西西安710049

出　　处：《西北农林科技大学学报（自然科学版）》2009年第6期151-157,共7页Journal of Northwest A&F University(Natural Science Edition)

基　　金：陕西省自然科学基础研究计划项目(2005C259)

摘　　要：【目的】研究解磷细菌和丛枝菌根真菌同时接种,对低磷土壤养分利用的影响。【方法】采用盆栽试验,通过测定生长于石灰性低磷土壤上红三叶草地上部的生物量及氮、磷营养吸收和根际酸性磷酸酶活性,研究丛枝菌根真菌、4种解磷细菌(编号分别为B1、B2、B3和B4)单独接种及同时接种对石灰性低磷土壤植物生长及氮、磷养分利用的影响。【结果】在石灰性低磷土壤条件下,4种解磷细菌单独接种对红三叶草植株地上部的生长和总吸磷量均无促进作用;单独接种丛枝菌根菌或不同解磷细菌与丛枝菌根真菌同时接种,均能显著提高红三叶草地上部干质量和总吸氮、磷量;在同时接种处理中,解磷细菌B4与丛枝菌根真菌间的相互作用对红三叶草的生长和氮、磷营养吸收具有明显的协同效应,能显著提高红三叶草地上部干质量、总吸氮和吸磷量及红三叶草根际酸性磷酸酶的活性。【结论】在石灰性低磷土壤中,解磷细菌与丛枝菌根真菌同时接种,对植物的氮、磷养分吸收具有明显的互作效应,但这种互作效应与解磷细菌和丛枝菌根真菌的种类有关,解磷细菌B4与丛枝菌根菌同时接种效果最佳。【Objective】The purpose of this article is to investigate the effect of co-inoculation with arbuscular mycorrhizal fungi（Glomus mosseae） and different bacteria on phosphate-solubilizing in a low phosphorus calcareous soil.【Method】The red clover（Trifolium pratense） was seeded in the pot with the soil single inoculated with arbuscular mycorrhizal fungus（Glomus mosseae strain）,single inoculated with four different phosphate（P）-solubilizing bacteria（numbered B1,B2,B3 and B4,separterly） or inoculation with both the phosphate（P）-solubilizing bacteria and arbuscular mycorrhizal fungus.The shoot dry matter yields,nitrogen and phosphorus nutrition and acid phosphatase activity of red clover（Trifolium pratense） were analyzed to value the contribution of these microbes,especially for phosphate-solubilizing bacteria to the phosphorus uptake of red clover in a low phosphorus soil.【Result】Single inoculation with the P-solubilizing bacteria had no effect on the shoot yields,nitrogen and total phosphorus uptake of red clover.Single inoculation with Glomus mosseae and co-inoculation with arbuscular mycorrhizal fungus and the P-solubilizing bacteria treatments showed higher shoot yields,total nitrogen and phosphorus uptake than un-inoculated controls or single inoculation with the P-solubilizing bacteria.There was a synergistic effect between B4 and arbuscular mycorrhizal fungi,which could enhance the shoot dry matter yields,total nitrogen and phosphorus uptake of red clover significantly,and promote the acid phosphatase activity in the rhizosphere.【Conclusion】Glomus mosseae could help the release of insoluble P in the calcareous soil.This effect could be enhanced significantly by co-inoculation with Glomus mosseae and the P-solubilizing bacteria.However,the synergism was related to the thpe of bacteria.The inoculation treatment with arbuscular mycorrhizal fungi（Glomus mosseae） and B4 had the best effect on the growth and nutrients uptake of red clover in a low phosphorus calcareous s

关 键 词：丛枝菌根真菌 解磷细菌 三叶草 酸性磷酸酶 

分 类 号：S144.9[农业科学—肥料学]