HPLC法测定四逆汤中甘草次酸的含量  

Determination of Enoxolone in Sini Decociton With HPLC Method

作　　者：陈静[1] 刘玉兰[2] 刘世坤[2] 

机构地区：[1]湘潭市第一人民医院,湖南湘潭411000 [2]中南大学湘雅三医院,湖南长沙410000

出　　处：《中医药导报》2009年第5期84-85,共2页Guiding Journal of Traditional Chinese Medicine and Pharmacy

摘　　要：目的:建立以高效液相色谱法测定四逆汤中甘草次酸含量的方法。方法:色谱柱:Kromasil C18柱(150mm×4.6mm,5μm),流动相:3.6%乙酸-甲醇(20∶80),流速1.0mL·min-1,检测波长:254nm,柱温:30℃。结果:甘草次酸的进样量在0.01-2.5μg(r=0.9994)范围内与峰面积积分值线性关系良好。平均回收率分别为97.70%(RSD%=2.36%)。结论:本方法简便、准确、分离效果好、线性范围宽、灵敏度高,为四逆汤及甘草制剂的质量控制提供了又一个考察指标。Objective： To establish HPLC method for the determination of enoxolone in sini decociton. Methods： The chromatographic coditions cosisted of Kromasil C18 column（150 mm ×4.6 mm,5 um） with mobile phase of 3.6% acetic acidmethanol（20：80） at flow rate of 1.0 mL·min^-1,the detection wavelength was 254 nm, column temperature：30 ℃. Results： The sample size of enoxolone showed a linear relationship with peak area at the range of 0.01-2.5ug（r=0.9994）,the average recovery was 97.70%（RSD%=2.36%）. Conclusion： The method is simple, accurate with good separating result and high sensible, that supplies a new target for quality control of sini decociton and radix glycyrrhizae preparation.

关 键 词：HPLC法 四逆汤 甘草次酸 

分 类 号：R927.2[医药卫生—药学]