粪便中戊型肝炎病毒散发株的核酸序列分析  

Nucleic acid sequence analysis of the sporadic hepatitis E virus strains isolated from stool samples

作　　者：魏绍静[1] Peter Walsh 唐漾波[1] 董惠卿[1] 蔡晓莉[1] 

机构地区：[1]广州市传染病医院 [2]香港理工大学

出　　处：《中华传染病杂志》1998年第2期82-84,共3页Chinese Journal of Infectious Diseases

基　　金：香港政府94-Chroucher基金;广州市科委资助

摘　　要：目的了解粪便中戊型肝炎病毒（ＨＥＶ）散发株的核酸序列变异情况。方法用逆转录－套式－聚合酶链反应（ＲＴ－Ｎｅｓｔｅｄ－ＰＣＲ）检测了８例广州地区散发性戊型肝炎患者粪便中的ＨＥＶＲＮＡ，３例阳性。对阳性ＰＣＲ产物的２３８个碱基进行了基因克隆和核酸序列分析，并与已报道的ＨＥＶ序列进行比较。结果本地区３株ＨＥＶ与缅甸株（Ｂ）、巴基斯坦株（Ｐ）、墨西哥株（Ｍ）、中国新疆株（Ｃｈ１．１）和广州血清株（Ｇ－９）的核苷酸和氨基酸的同源性均值分别为８０．６７％和８８．６０（Ｂ）、８１．２５％和８９．２０％（Ｐ）、７７．４５％和８４．８１％（Ｍ）、８１．２５％和８９．２０％（Ｃ）、９７．８５％和９６．２０％（Ｇ）。结论本组３株ＨＥＶ有一定程度的变异。Objective In order to investigate the genomutation of sporadic HEV strains we analyzed the nucleotide sequence of sporadic HEV strains in Guangzhou.Methods RT Nested PCR was used to determinate HEV RNA in stool samples from 8 patients with sporadic hepatitis E. Results 3 stool samples were positive for HEV RNA, which were then hybridized、 recombinanted and sequenced. The homology of nucleic acid and amino acid sequences of 3 HEV strains in Guangzhou comparing with the Burmese、Pakistani、Mexican、Chinese Ch1.1 and G 9 strains were 80.67% and 88.60(B)、81.25% and 89.20%(P)、77.45% and 84.81%(M)、81.25% and 89.20%(C)、97.85% and 96.20% (G) respectively.Conclusion The results suggested that 3 strains of HEV in Guangzhou had genomutation to a certain extent.

关 键 词：戊型肝炎病毒 RNA 核酸序列分析 粪便 

分 类 号：R512.630.4[医药卫生—内科学] R373.2[医药卫生—临床医学]