溶菌酶与矿物粉尘反应行为的模拟研究  

Simulated Study on the Reaction between Lysozyme and Fiber Dusts

作　　者：白进伟[1] 迟燕华[1] 胡亚敏[1] 

机构地区：[1]西南科技大学材料科学与工程学院,四川绵阳621010

出　　处：《西南科技大学学报》2009年第2期70-73,共4页Journal of Southwest University of Science and Technology

基　　金：国家自然科学基金(400720201);教育部科学技术研究重点项目(02126)资助

摘　　要：对3个地区的矿物粉尘做了XRF分析及其与溶菌酶(Lys)的反应实验,结果显示Lys与样品中的物质进行了反应。在模拟样品中元素离子与Lys反应的UV-V is、荧光光谱研究中发现,37℃下Lys与样品中的元素离子Fe3+、Pb2+的作用很明显。表现在紫外区Lys-Fe3+复合物吸光度增强,Lys-Pb2+复合物吸收峰红移13 nm。荧光区Lys-Fe3+复合物在λem450 nm处荧光强度增强,荧光峰红移30 nm,Lys-Pb2+复合物在λem725 nm处荧光强度增强,荧光峰红移205 nm。Fiber dusts of three different areas were analyzed by XRF and samples were reacted with Lysozyme. The results show that Lysozyme has reacted with the substances in the samples. The UV-Vis and Fluorescent Spectra study was conducted on the reaction between the simulated ion and Lys, which found that the reaction between Lysozyme and element Fe^3＋ , Pb^2＋ in the samples was obvious at 37℃. In UV- vis spectra the Lys-Fe^3＋ absorption was strengthened and the Lys-Pb^2＋ maximum absorption peak red shifted 13 nm. In Fluorescent spectra, the Lys-Fe3＋maximum fluorescence intensity appeared at the λem 450 nm and the fluorescence peak red shifted 30 nm, the Lys-Pb^2＋ maximum fluorescence intensity appeared at the λem 725 nm and the fluorescence peak red shifted 205nm.

关 键 词：溶菌酶 矿物粉尘 UV—Vis光谱 荧光光谱 

分 类 号：Q556[生物学—生物化学]