负载供者抗原的第三方树突状细胞延长大鼠移植心脏存活  

Effect of the Third Party Immature Dendritic Cells Loaded with Donor Antigen on Survival of Rat Cardiac Allograft

作　　者：崔志刚[1] 汪泽厚[2] 岳明[1] 叶钢[3] 黄赤兵[3] 张艮甫[3] 

机构地区：[1]解放军第306医院泌尿外科,北京100101 [2]解放军空军总医院泌尿外科,北京100036 [3]第三军医大学附属新桥医院泌尿外科,重庆400037

出　　处：《中国比较医学杂志》2009年第5期1-5,共5页Chinese Journal of Comparative Medicine

摘　　要：目的观察负载供者抗原第三方未成熟DC对大鼠同种异体移植心脏存活期的影响。方法培养骨髓来源第三方大鼠未成熟DC,负载供者抗原,CTLA-4 Ig致耐受处理。建立大鼠心脏腹部移植模型,A组:心脏移植;B组:心脏移植,术前输注供者源未成熟DC;C组:心脏移植,术前输注第三方未成熟DC;D组:心脏移植,术前输注负载供者抗原致耐受第三方未成熟DC。分别行移植心病理、IFNγmRNA表达和移植心存活时间观察。结果D组的移植心病理改变轻,IFNγmRNA表达下调,移植心脏存活时间显著延长。结论负载供者抗原第三方致耐受未成熟DC能够诱导供者特异性免疫耐受,明显延长大鼠移植心脏存活时间。Objective To investigate the effects of the third party immature dendritic cells（DCs） loaded with donor antigen and CTLA-4 Ig on survival of rat cardiac allograft. Methods The third party immature DCs were cultured from rat bone marrow, loaded with donor antigen and CTLA-4 Ig. the model of rat heart abdominal transplantation was established. Animals were divided into four groups. Group A received no DCs. Group B received donor derived immature DCs. Group C received the third party immature DCs alone. Group D received the third party immature DCs loaded with donor antigen and CTLA-4 Ig. The histology and the IFNγmRNA expression of intragraft were tested respectively by Light examinations and RT-PCR. Transplanted hearts were monitored daily by direct palpation. Results The histology changed little, The mRNA expression of IFNγ was declined and the survival of rat cardiac allograft was significantly prolonged in group4. Conclusions The third party immature DCs with donor antigen and CTLA-4 Ig can induce Ag-specific immune tolerance and significantly prolong survival of rat cardiac allograft.

关 键 词：树突状细胞 移植 免疫耐受 

分 类 号：R392.4[医药卫生—免疫学]