近交系DA大鼠遗传学质量  

Detection of Genes in the New Inbred Strain DA Rat

作　　者：林彦[1] 唐小玲[2] 王洪[3] 岳秉飞[3] 王海霞[1] 

机构地区：[1]哈尔滨医科大学第二附属医院动物实验中心,哈尔滨150081 [2]哈药集团制药总厂,哈尔滨150081 [3]中国药品生物制品检定所,北京100020

出　　处：《中国比较医学杂志》2009年第5期59-61,F0003,共4页Chinese Journal of Comparative Medicine

基　　金：黑龙江省科技攻关项目(项目编号PC07S07);黑龙省教育厅科学技术研究面上项目计划(项目编号11531175)

摘　　要：目的通过鼠尾皮肤循环移植和生化标记法检测DA大鼠的生化标记基因,为动物实验的研究提供丰富的动物基因库。方法采用鼠尾皮肤循环移植法观察自体与异体皮片成活率,采用蛋白质与同工酶醋酸纤膜电泳法,对试验DA大鼠进行碱性磷酸酶等11个生化标记基因位点进行确定。结果DA大鼠自体与异体移植的皮片在100 d的观察期间全部成活,DA大鼠11个生化标记基因位点均为纯合的。结论DA大鼠生化位点的确定,奠定了DA大鼠的生化位点遗传概貌的理论基础,为进一步研究DA大鼠提供遗传学资料。Objective To detect the genes of inbred strain DA rat by skingrafting and genetic biolchemical labeling. Methods The survivalrate of self and Variant were observed by skingrafting, and 11 alleles, including Akp-1 and others, were assayed in the inbred DA rat by genetic biolchemical labeling technique. Result The free skin graft of self and Variant are all alive during 100 days,and 11 alleles,including Akp-1 and others are all homozygous. Conclusion The definite of biochemical locus establish the rationale of hereditary atlas and provid the invaluable resource for experimental animal research as well.

关 键 词：Dahl-R 基因 检测 

分 类 号：Q953[生物学—动物学]