巨噬细胞对掺锶聚磷酸钙降解行为影响的体外研究  

Degradation Studies of Strontium-doped Calcium Polyphosphate with Macrophages in Vitro

作　　者：陈峰[1] 田猛[1] 秦滢杰[1] 张小华[1] 余喜讯[1] 万昌秀[1] 

机构地区：[1]四川大学高分子科学与工程学院,四川成都610065

出　　处：《航天医学与医学工程》2009年第3期221-225,共5页Space Medicine & Medical Engineering

基　　金：国家自然科学基金资助项目(30870614;30870616)

摘　　要：目的通过设计巨噬细胞在体外对SCPP的降解实验,为骨组织工程支架材料掺锶聚磷酸钙(SCPP)体内降解规律的研究提供基础和参考。方法制备SCPP支架材料,将该材料与巨噬细胞RAW264.7培养6周以研究细胞介导的降解。用扫描电镜(SEM)观察细胞及材料形貌;复合电极测量培养液pH值变化和用等离子体发射光谱检测培养液中的Ca2+、磷(Pi)、Sr2+浓度。结果SEM结果表明,培养细胞后材料孔径与粒径均减小,表面粗糙度增大,降解显著;细胞组培养液pH值在6.87～7.17之间,明显低于对照组;细胞组培养液中Ca2+、Pi、Sr2+释放量明显高于对照组。细胞介导的降解速率约为对照组的5倍,且Sr2+浓度在正常生理范围内。结论在SCPP降解过程中,巨噬细胞加速了SCPP的降解,并在降解过程中起到主导作用。Objective To lay the foundation and provide reference to investigate the degradation of SCPP with the study of degradation of strontium-doped calcium polyphosphate（SCPP） for bone tissue engineering in vivo. Methods Cell-mediated biodegradation of SCPP was cultured with a macrophage line （RAW264.7） for 6 weeks as cell group and SCPP was cultured without cells as control. SCPP and macrophages were imaged with scanning electron microscopy （SEM） ; the pH value of the culture medium at each time-point was determined with a pH meter and the calcium, phosphate and strontium released from materials to the culture medium were analyzed with inductive coupled plasma atomic emission spectroscopy. Results The results of SEM measurements showed that in cell group the pore and particle size of materials decreased obviously and a more roughness surface was observed, indicating materials degraded remarkably. The pH value of culture medium in cell group was 6.87 -7.17 less than control group. The quantities of calcium, phosphate and strontium released to culture medium in cell group were significantly higher than control group respectively. Sr^2＋ concentration was still in the normal physiological range. The rate of cell-mediated biodegradation was about five times more rapidly than control group. Conclusion In degradation process of SCPP, all these results prove macrophage accelerates the degradation of SCPP and show that cell-mediated biodegradation plays a dominant role in the degradation process of SCPP.

关 键 词：锶 掺锶聚磷酸钙 降解 巨噬细胞 

分 类 号：R318.08[医药卫生—生物医学工程]