云斑尖塘鳢、线纹尖塘鳢及其杂交子一代间的遗传关系  

Studies on genetic relationship between Oxyeleotris marmoratus,Oxyeleotris lineolatus and their first filial generation

作　　者：范小勇[1] 王茜[1] 齐兴柱[1] 骆剑[1] 王小刚[1] 尹绍武[1] 陈国华[1] 吴光明 

机构地区：[1]海南大学海洋学院,海南省热带水生生物技术重点实验室,热带生物资源教育部重点实验室,海口570228 [2]海南断山渔业有限公司,三亚572013

出　　处：《海洋渔业》2009年第2期128-133,共6页Marine Fisheries

基　　金：海南省重点科技计划项目资助(070119);三亚市重点科技项目(080115);海南大学重点学科建设项目资助

摘　　要：应用RAPD技术研究云斑尖塘鳢、线纹尖塘鳢及其杂交子一代(F1)间的遗传关系。筛选得到25个随机引物均在亲本和杂交子一代间呈现多态,遗传相似性指数和遗传距离的计算结果显示,正交F1(云斑尖塘鳢♂×线纹尖塘鳢♀)与母本平均遗传相似系数(0.7090)高于与父本平均遗传相似系数(0.5948);反交F1(云斑尖塘鳢♀×线纹尖塘鳢♂)与母本平均遗传相似系数(0.6301)也高于与父本平均遗传相似系数(0.6089)。说明正反交子代在遗传关系上均偏向各自的母本,对扩增带和聚类图的分析均得到这一结果,表明F1所接受的双亲遗传物质并非完全对等的,所获得的遗传信息可能更多来自母本。By means of RAPD technique, this paper deals with the study on the heterosis and genetic relationships between the Oxyeleotris marmoratus, Oxyeleotris lineolatus and their first filial generation （ F1 ）. 25 primers randomly selected from 100 primers all revealed genetic polymorphism between parents and F1. Accroding to the calculation based on the data of genetic similarity indices and genetic distance, it came to the conclusion that the average genetic similarity coefficient between positive hybrid F1 （Oxyeleotris marmoratus × Oxyeleotris lineolatus 9 ） and female parent （ 0. 709 0 ） was higher than that between F1 and male parent （0. 594 8 ）. And the average genetic similarity coefficient between negative hybrid F1 （Oxyeleotris marmoratus ♀× Oxyeleotris lineolatus ♂ ） and female parent （0. 630 1 ） was also higher than that between F1 and male parent （0. 608 9）. These results illustrated that the offspring of both positive and negative hybrid were more similar to their female parent. The same results have been summarized according to the assay by means of RAPD technique and UPGMA tree. At all status hybrids F1 did not derive equivalent genetic material from parents, but more from the female parent.

关 键 词：云斑尖塘鳢 线纹尖塘鳢 杂交 遗传关系 杂种优势 

分 类 号：S961.5[农业科学—水产养殖]