苦瓜水提取物对诱变剂致突变拮抗作用  

Antagonistic effects of water extracts from Momordica charantia on mutagens

作　　者：曹向宇[1] 曾猛[2] 刘剑利[1] 李其久[1] 常浩[3] 

机构地区：[1]辽宁大学生命科学院生物化学教研室,沈阳110036 [2]天津市环境保护研究院 [3]沈阳三生制药有限责任公司

出　　处：《中国公共卫生》2009年第6期658-659,共2页Chinese Journal of Public Health

基　　金：辽宁省科技厅科技攻关项目(2004205001)

摘　　要：目的探讨苦瓜水提取物的抗突变作用。方法应用彗星实验及Ames实验对苦瓜水提取物进行抗突变研究。结果彗星试验2.5,5.0,10.0g/(kg.bw)3个剂量组与阳性对照组比较,差异有统计学意义(P<0.05),减少环磷酰胺(CP)引起的细胞DNA损伤;Ames试验5000,1667,556μg/皿3个剂量组在+/-S9的条件下,均能不同程度地抑制二氨基芴(2-AF)、敌克松(Dexon)引起的TA98、TA100回变菌落数的增加。结论在本实验条件下,苦瓜水提取物具有明显的抗突变作用。Objective To study the anti-mutagenicity of water extracts from Momordica charantia. Methods The comet assay and Ames test were used to detect the anti-mutagenicity of water extracts from Momordica charantia. Results The comet assay results showed that there was significant difference between the negative control and the treated groups in dose of 2. 5,5.0,10. 0g/kg · bw（ P 〈 0. 05 ）. The water extracts from Momordica charantia could reduce the damage of cell caused by cyclophosphamide. The Ames test results showed that the increasing of the frqeuency of revertant colonies of TA98 ,TA100 treated with positive mutagens could be inhibited by the 5000,1667,556μg/plate of water extracts from Momordica charantia with or without S9. Conclusion The water extracts from Momordica charantia can inhibit mutagenesis caused by mutagens in the test.

关 键 词：苦瓜水提取物 抗突变作用 彗星实验 AMES实验 

分 类 号：R114[医药卫生—卫生毒理学]