抗砷细胞内荧光物质激光共聚焦显微镜检测  

Detection of fluorescent material in arsenic-resistant cells with LSCM

作　　者：郭淑丽[1] 罗先道[1] 杨丽[1] 程建兵[1] 杨磊[1,2] 

机构地区：[1]石河子大学医学院一新疆地方病与民族高发病省部共建重点实验室生化教研室,新疆石河子832002 [2]杭州师范大学医药卫生管理学院

出　　处：《中国公共卫生》2009年第6期710-711,共2页Chinese Journal of Public Health

基　　金：国家自然科学基金(30560129)

摘　　要：目的应用激光共聚焦显微镜检测抗砷细胞内荧光物质含量。方法用荧光染料Rhodamine-123对抗砷细胞进行荧光染色30min,分别用维拉帕米(Verapamil)、亚砷酸钠处理细胞,单纯Rhodamine-123的抗砷细胞为对照组。应用激光共聚焦显微镜采集Rhodamine-123的荧光图像动态序列,并且记录12,24,36,48,60h等不同时段细胞内荧光强度。结果实验组细胞染色48h后,Verapamil实验组荧光强度分别为(38.940±0.3648),(38.153±0.533),均明显高于同时间段对照组的荧光强度,差异均有统计学意义(均P<0.05)。结论激光共聚焦显微成像技术可用于抗砷细胞拮抗效果实时定量检测。Objective To detect the fluorescent material within arsenic-resistant cells by laser scanning confocal micro- scope（LSCM）. Methods The arsenic-resistant cells were incubated with fluorescent dyes Rhodamine-123 for 30 minutes. The cells of test group were co-incubated with Verapamil and NaSO~ ,respectively. The cells of control group were merely administrated with Rhodamine-123. Fluorescence image acquisition of Rhodamine - 123~ dynamic series were executed with LSCM, and the concentration fluorescence intracell at different time （12,24,36,48,64h） was recorded. Results After fluo- rescence staining for 48h,the fluorescence intensity of the test group was 38.94 ±0. 3648 and 38. 153 ±0. 533 for Verapamil and NaASO2 treatment. The intensities were significantly higher than that of the synchronous control group. Conclusion The LSCM could be used to detect fluorescent material in arsenic-resistant cells timely and quantitively.

关 键 词：抗砷 活细胞 荧光染料 激光共聚焦显微镜 

分 类 号：R995[医药卫生—毒理学]