噬菌体分型技术和脉冲场凝胶电泳技术对肠炎沙门菌分型的比较  

Comparison of phage typing and pulsed-field gel electrophoresis in typing of Salmonella enteritidis

作　　者：林一曼[1] 兰全学[1] 石晓路[1] 刘涛[1] 扈庆华[1] 

机构地区：[1]广东省深圳市疾病预防控制中心,广东深圳518020

出　　处：《中国卫生检验杂志》2009年第5期998-999,1048,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:比较噬菌体分型技术和脉冲场凝胶电泳(PFGE)技术对肠炎沙门菌分型的分辨力。方法:对从病人及食物分离出的83株肠炎沙门菌,用9种沙门菌噬菌体进行分型,以XbaI作为限制性内切酶进行PFGE分型。结果:噬菌体将83株肠炎沙门菌分为4种型,PFGE将83株肠炎沙门菌分为19种型。同种噬菌体型的肠炎沙门菌用PFGE可分出更多的型。结论:在肠炎沙门菌的分型上,PFGE比噬菌体分型显示了更高的分辨力,PFGE技术仍然是目前肠炎沙门菌分型的"金标准",它更适合于食物中毒的分子流行病学调查。Objective：To compare the discriminatory ability between phage typing and pulsed -field gel electrophoresis （PFGE） in Salmonella enteritidis. Methods：83 strains of Salmonella enteritidis from patients and food were typed by nine Salmonella phages and PFGE with XbaI as the restriction enzyme. Results：83 strains of Salmonella enteritidis were divided into 4 types by phage typing and 19 types by PFGE, respectively. The same phage type of Salmonella enteritidis can be divided into different types by PFGE. Conclusion：PFGE shows higher discriminatory ability in the subtype of Salmonella enteritidis than phage typing. PFGE is still the ＂gold standard＂ for typing Salmonella enteritidis. PFGE is suitable for molecular epidemiological investigation for food poisoning.

关 键 词：噬菌体分型 脉冲场凝胶电泳 肠炎沙门菌 

分 类 号：R378.22[医药卫生—病原生物学]