选择性培养基对-20℃冷冻损伤致泻大肠埃希菌生长的影响  

Influence of select medium on survival of freeze-injured diarrheogenic Escherichia coli at-20℃

作　　者：张勇[1] 刘衡川[2] 殷强仲[2] 曾华书[1] 刘哲民[1] 

机构地区：[1]广东省深圳市福田区疾病预防控制中心检验科,广东深圳518040 [2]四川大学华西公共卫生学院,成都610041

出　　处：《中国卫生检验杂志》2009年第5期1055-1057,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:分析常规检测中使用的伊红美蓝琼脂(EMB)、乳糖胆盐发酵培养基(LBB)、麦康凯琼脂(MCA)、肠道菌增菌肉汤(EEB)对-20℃冷冻损伤致泻大肠埃希菌生长的影响。方法:选用4株致泻大肠埃希菌作为研究菌株,-20℃冷冻贮存不同时间后,分析EMB、LBB、MCA、EEB对其生长的影响。结果:1.除EMB培养基外,LBB、MCA、EEB培养基可抑制-20℃冷冻损伤致泻大肠埃希菌的生长,MCA、EEB的抑制作用大于LBB的抑制作用;2.不同的菌株对冷冻的敏感性不同。在不同的冷冻时间,菌株的死亡率、损伤率不同。结论:常规检测中使用的LBB、MCA、EEB可抑制-20℃冷冻损伤致泻大肠埃希菌的生长。Objective ： To study the effects of the EMB, LBB, MCA and EEB on the survival of the freeze - injured diarrheogenic Escherchia coli at - 20℃. Methods ： The influence of the EMB, LBB, MCA and EEB on the survival of four freeze - injured diarrheogenic Escherchia coli at -20℃ storage was studied. Results：l. Except for EMB, LBB, MCA and EEB inhibited the survival of the freeze -injured diarrheogenic Escherchia coli at -20℃, and MCA and EEB had more inhibition effects than LBB. 2. Different types of bacteria had different sensitivities to freezing. Diarrheogenic Escherichia coli had different proportion of freeze - injury and freeze - mortality during different frozen storage. Conclusions：The LBB, MCA and EEB can inhibit the survival of the freeze - injured diarrheogenic Escherchia coli at - 20℃.

关 键 词：冷冻损伤 致泻大肠埃希菌 选择性培养基 

分 类 号：R446.5[医药卫生—诊断学]