移植pSVPoMcat微基因修饰雪旺氏细胞对脊髓损伤后细胞凋亡的影响  

Effect of Microgene pSVPoMcat Implanted to Modify Genetically Schwann Cells on Apoptosis After Spinal Cord Injury

作　　者：陈礼刚 高立达[1,2,3] 曾凡俊[1,2,3] 胡威夷 毛伯镛[1,2,3] 陈俊杰 赖立辉[1,2,3] 李开慧[1,2,3] 卢敏[1,2,3] 

机构地区：[1]成都军区总医院神经外科 [2]华西医科大学附属第一医院神经外科 [3]华西医科大学基础医学重组DNA研究室

出　　处：《中华创伤杂志》1998年第3期157-159,共3页Chinese Journal of Trauma

摘　　要：目的探讨ｐＳＶＰｏＭｃａｔ微基因修饰雪旺氏细胞（ＳＣ）移植对脊髓损伤（ＳＣＩ）后细胞凋亡的作用。方法将实验动物分为ｐＳＶＰｏＭｃａｔ微基因修饰ＳＣ组（Ａ组），ＳＣ移植组（Ｂ组），损伤对照组（Ｃ组）。移植后１２周，对ＳＣＩ区的脊髓组织切片进行细胞凋亡的检测（甲基绿－派诺宁染色法和荧光原位标记法）以及Ｂｃ１～２和Ｆａｓ蛋白表达的测定（免疫组化法）。结果在Ａ、Ｂ、Ｃ组中，均发现凋亡细胞及Ｂｃ１～２和Ｆａｓ蛋白阳性表达。体视学计量发现，细胞凋亡率为Ｃ＞Ｂ＞Ａ；Ｂｃ１～２蛋白阳性表达顺序为Ａ＞Ｂ＞Ｃ；Ｆａｓ蛋白阳性表达顺序为Ｃ＞Ｂ＞Ａ。结论ｐＳＶＰｏＭｃａｔ微基因修饰ＳＣ移植能抑制ＳＣＩ后的细胞凋亡。Aim To study the effect of microgene pSVPoMcat implanted to genetically modify Schwann cells (SC) on apoptosis after spinal cord injury (SCI). Methods Experimental animals were divided into three groups: the group of microgene pSVPoMcat implanted to genetically modify SC (group A), SC implanted group (group B) and the control group (group C). The apoptosis of spinal slice from the injured spinal cord were examined by the methods of methye green, terminal deoxynucleotidyl and the dUTP nick end labelling technique, and the expression of Bc1 2 and Fas protein were measured immunohistochemically 12 weeks post operation. Result The rate of apoptosis sequence, the strong expression sequence of Bc1 2 and Fas protein expression were found in all three groups, but C>B>A ( P <0.05), A>B>C ( P <0.05), and C>B>A ( P <0.05), respectively. Conclusion It indicates that the pSVPoMcat implanted to genetically modify SC can inhibit the apoptosis, and trauma can induce apoptosis after SCI.

关 键 词：细胞凋亡 脊髓损伤 基因修饰 雪旺氏细胞 

分 类 号：R651.2[医药卫生—外科学]