骨形成蛋白复合生物活性陶瓷修复骨缺损时血管的变化  

Vessels' Change After Implanted with Bone Morphogenetic Protein Combined with Bioactive Glass Ceramics in Bone Defects

作　　者：乔王平 高玉好 杨连甲[1,2] 

机构地区：[1]解放军第二八九医院骨科 [2]第四军医大学附属口腔医学院组织病理教研室

出　　处：《中华创伤杂志》1998年第3期172-174,共3页Chinese Journal of Trauma

摘　　要：目的研究骨形成蛋白（ＢＭＰ）复合生物活性陶瓷（ＢＧＣ）修复骨缺损时血管的变化。方法采用血管铸型方法，取大白鼠８只，随机分为４组。在麻醉下造成股骨中段１ｃｍ×０．５ｃｍ缺损，然后植入ＢＭＰ－ＢＧＣ复合材料。术后不同时相分别处死动物，观察缺损区微细血管的变化。结果植入ＢＭＰ－ＢＧＣ复合物后１周，在颗粒之间可见血管芽和新生血管形成。植入后２周，更多的血管形成，并构成血管网。植入后３周，血管网仍可见，有些血管向ＢＧＣ颗粒孔中生长。术后４周，骨缺损已愈合。与对照组相比，在ＢＭＰ－ＢＧＣ植入组中，新生血管的数量在４个时间点上都比对照组多，新骨形成也明显较早。结论ＢＭＰ不仅可诱导间质细胞向成骨细胞分化，而且也可以刺激血管的形成。这些新生血管既为骨缺损的愈合带来营养物质。Aim To study the changes of the microvascular architecture during implantation of bone morphogenetic protein (BMP) combined with bioactive glass ceramics (BGC) in bone defects. Methods The experimental rats were made femur bone defects for 1.0cm×0.5cm under anaesthetic state and then were planted with the composites of BMP and BGC. Then the rats were killed at different time points and the changes of microvessels in bone defects were observed. Results One week after the implantation of BMG BGC composites, vascular buds and newly formed blood vessels were found between BMP BGC granules. Two weeks later, many new vessels were observed, forming a network of blood vessels. Three weeks later, the network still existed and some vessels grew into the pores of BGC. Four weeks later, the defects were healed. Compared with the control group, the newly formed blood vessels in BMP BGC groups increased in number at four time points and the new bones could be observed obviously early. Conclusions BMP might not only induce differentiation of mesenchymal cells, but also stimulate the formation of blood vessels. These new vessels offer nutrition for the healing of bone defects and provide more target cells for bone formation.

关 键 词：骨形成蛋白 生物活性陶瓷 骨缺损 血管 

分 类 号：R687[医药卫生—骨科学] R318.08[医药卫生—外科学]