解脲脲原体对红霉素体外耐药性与ermB基因相关性研究  

Study on the relationship between erythromycin sensitivity and ermB gene in Ureaplasma urealyticum

作　　者：叶庭路[1] 陆春[1] 卢荣标[1] 马寒[1] 陈传杰[1] 赖维[1] 朱国兴[1] 陈剑[1] 

机构地区：[1]中山大学附属第三医院皮肤性病科,广州510630

出　　处：《中华微生物学和免疫学杂志》2009年第5期472-475,共4页Chinese Journal of Microbiology and Immunology

基　　金：广东省自然科学基金资助（8151008901000108）;广东省科学计划项目基金资助（2007B030502013）

摘　　要：目的探讨解脲脲原体（Uu）临床株对红霉素体外耐药性与ermB耐药基因之间的关系。方法采用微量肉汤稀释法体外测定143株临床分离的池对红霉素的最低抑菌浓度（MIC），以MIC≥8μg／ml为耐药判读标准；设计引物扩增ermB基因，并以多条带抗原基因为靶位设计引物对批进行生物学分群。结果跳对红霉素MIC范围为≤0．125μg/ml至≥128μg／ml，MIC50为16μg／ml，MIC90≥128μg／ml，耐药率为64．38％。ermB基因总的阳性率为27．97％，主要分布在MIC≥8μg/ml的菌株。两生物群之间不存在红霉素耐药性及ermB基因阳性率的差异。结论ermB基因可能是介导沈对红霉素耐药的基因，两生物群在对红霉素耐药性机制的差异需进一步研究。Objective To study the relationship between erythromycin sensitivity and ermB gene in 143 Ureaplasrna urealyticum （Uu） clinical isolates. Methods We detected the minimum inhabit concentrations （MICs） of Uu to erythromycin by broth dilution method and MIC ≥ 8 μg/ml was used as standard concentration of resistance to erythromycin. Polymerase chain reaction was used to detect the ermB gene and biotype Uu with primers based on multi-band antigen gene. Results The MICs, MIC50, MIC90 of Uu to erythromycin were ≤0. 125 μg/ml to ≥ 128 μg/ml, 16 μg/ml, and ≥ 128 μg/ml, respectively, with a high resistance rate of 64.38%. ermB gene, which was mainly detected in Uu with MIC ≥8 μg/ml, was positively detected in 40 out of 143 Uu strains （ 27.97% ）. No significant differences of the resistance to erythromycin and positive rate of ermB gene were found between the two biovars in the study . Conclusion ermB gene may probably be one of the important genes conferring resistance to erythromycin in Uu. Further studies are needed to discover the difference of resistance and mechanism of erythromycin between the two biovars.

关 键 词：解脲脲原体 红霉素 药物敏感试验 ERMB基因 

分 类 号：R446.5[医药卫生—诊断学] R450[医药卫生—临床医学]