多质粒共转染建立Cav1.3 L-型钙通道的研究模型  

A study on the model of Calcium Ion Channel by co-transfection of multi-Plasmid DNA recombinant

作　　者：强华[1] 魏峰[1] 张爱峰[1] 史瑞明[1] 孙超峰[1] 马爱群[1] 

机构地区：[1]西安交通大学医学院第一附属医院心内科,教育部环境与疾病相关基因重点实验室离子通道病室,西安710061

出　　处：《陕西医学杂志》2009年第6期643-644,647,共3页Shaanxi Medical Journal

基　　金：国家自然科学基金资助项目(No:30800434)

摘　　要：目的:提高多质粒转染效率,建立钙离子通道的研究模型。方法:在传统的脂质体转染过程中,通过不断调整细胞密度、转染质粒DNA的量,将含Cav1.3L-型钙通道相关亚基的质粒DNA转染至HEK293T细胞,采用全细胞膜片钳技术检测Cav1.3L-型钙通道的电流表达。结果:转染后的HEK293T细胞在荧光显微镜下可见明显的绿色荧光;全细胞膜片钳技术检测出Cav1.3 L-型钙通道电流的表达。结论:通过调整细胞密度和转染质粒DNA的量,可以提高脂质体介导多质粒共转染HEK293T细胞的效率,建立钙离子通道的研究模型。Objective： To enhance the efficiency of multi-plasmid co-transfection and establish a research model of calcium ion channel. Methods ： In the process of conventional transfection using lipofectamin, plasmid DNA recombinant with subunits related to Cavl. 3 L-type calcium channel was co-transfected into HEK293T cells by adjusting the cell density and quantity of plasmid DNA. The current expression of Cavl. 3 L-type calcium channel was detected by whole-cell patch-clamp technique. Results： The transfeeted HEK293T cells displayed green fluorescence under the fluorescence microscope. And the current expression of Cavl. 3L-type calcium channel has also been detected. Conclusions： By adjusting the cell density and quantity of plasmid DNA, the efficiency of multi-plasmid co-transtection into HEK293T cells mediated by lipofeetamin is efficiently enhanced and the study model of Cavl. 3 L-type calcium channel is also established.

关 键 词：钙通道 L型 基因 脂质体 @人胚胎肾 293T细胞 

分 类 号：R972[医药卫生—药品] R965[医药卫生—药学]